Connected on 2012-02-08 19:00:00 from , Western Australia, Australia
- 6:03pm
- Bugscope Team sample is pumping down
- Bugscope Team almost there...
- Bugscope Team it's like 8:07 a.m. tomorrow in Perth
- 6:10pm




- 6:16pm

- Bugscope Team we are ready to roll at any time now

- Bugscope Team cornicles are also called siphunculi
- 6:21pm

- 6:28pm

- Bugscope Team as if something you never heard of has to have two names
- Bugscope Team alright it is 6 1/2 here
- 6:33pm
- Bugscope Team I mean you can hardly see them, and they have names.
- Bugscope Team they're kind of like stern-mounted cannons
- Bugscope Team or dual exhaust in the aphid world
- 6:41pm
- Bugscope Team you're a student!

- Bugscope Team Welcome to Bugscope! Is everything, like, upside down there?
- Student You are upside down!
- Student I can't type here
- Bugscope Team haha maybe so
- Student CAn I drive the scope?
- Student I am psyched!
- Bugscope Team yeah I gave you control
- Bugscope Team although you're only a student
- Bugscope Team I believe you already moved the ant

- Bugscope Team here goes
- Bugscope Team Yay! Can you see the glossa?
- Bugscope Team the wasp tongue?
- Bugscope Team it's a delicacy
- Student I can see the glossa! pRETTY cool. Don't get this laptop


- Bugscope Team is it like ergonomic or something?
- Bugscope Team now you can see two palps and more of the mandible
- Student It is. Too good for me.
- Bugscope Team Kendra has one of those and I am totally at sea with it.
- Student It looks like things are working. The scientists in this room are chomping to take over the podium. Good thing I have a black belt.

- Bugscope Team haha take it easy down there
- Student I am using internet explorer. Will that be OK? I dont' have Firefox here.
- 6:46pm
- Bugscope Team yeah should be fine
- Student OK - I have to log off. People are giving me the eye. Looks like this is all working. What do you think NEed to try anything?
- Bugscope Team it all looks good there? I think if it's all working the only potential problem is the screen res.
- Student great samples.
- Student The resolution is good!
- Student ALl ok?
- Student ALL
- Bugscope Team We are fine Mademoiselle; new sample for your session in a coupla days.
- Student COOL! OK - gotta go mate. G'Day
- Student Thankks for the testing.
- Bugscope Team over and out!
- Student roger that
- Bugscope Team np
- Bugscope Team Bye!
- 5:56pm
- Bugscope Team ...
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- Bugscope Team ...
- Bugscope Team sample is pumping down
- 6:07pm
- Bugscope Team setting up


- 6:14pm



- 6:19pm



- 6:26pm




- Bugscope Team making presets...
- 6:31pm

- 6:37pm



- 6:43pm


- Bugscope Team Hello!
- Guest HI guys - i had trouble logging in from my password - so I am a guest
- Bugscope Team How are things on the ventral side of the world?
- Guest We will be starting in 15 minutes or so - i will start with the power point
- Bugscope Team you can try logging in again; didn't your password do the trick Monday?
- Bugscope Team this is a pretty gnarly looking mite
- 6:48pm
- Bugscope Team Hey does this ant look rightside up to you?



- Guest it looks great@!
- 6:54pm
- Bugscope Team this is the turret for the bedbug proboscis
- Guest that is gross
- Guest Good morning, Michele! I hope you are doing well.
- Bugscope Team Umesh!
- Guest Good evening, Scott!

- Bugscope Team Colonel Chas is here as well, a rare occasion
- Guest Hello Chas! Did you have many Facebook options.
- Guest The whole team is here! Yeah! A shout out to my family and Marlo for being there too. Hey to Jen and Mike!! They all encouraged me to come!! We will be starting soon@
- Bugscope Team Umesh, Chas' chauffeur dropped him off.
- Guest Hi Michele!
- Bugscope Team Would have been nice. My Apple stuff is doing well though
- Guest I will be back soon = starting with the presentation for a bit then online.
- Bugscope Team Great!
- Bugscope Team Awesome.
- Bugscope Team ...
- Bugscope Team ...
- Bugscope Team ...
- Guest Hi Kate!
- 6:59pm
- Guest People are excited so far!
- Bugscope Team Sweet. I will put the ant on and see if it looks like its rightside up to you.

- Guest This is your show, Michele! Drive the scope!
Bugscope Team Yo Dude I think she's talkin'

- 7:05pm
- Guest Wish we had bugs from down under.
- 7:16pm

- Guest Here we are. JUst finished with the PPT. Scot - can you drive the scope and show us something in partcular?
- Bugscope Team this is a bedbug, Michele, that you gave us
- Bugscope Team Scott just moved to the bedbug (cimex lectularious)
- Bugscope Team hope it wasn't your personal, like, pet
- Bugscope Team I'm sitting at the 'scope and Chas is on Chaos, next to the 'scope
- Bugscope Team This is zooming in on a ball joint at the base of the proboscis
- Guest it was caught by an exterminator
Bugscope Team ha Thanks for Clarifying!
- Guest what is this structure?
Bugscope Team this is the turret part of the head
- Bugscope Team The proboscis is a tube-like structure they use to suck blood through
- Bugscope Team the proboscis can turn forward and poke into your skin no problem
- Bugscope Team The spikey structures are setae, roughly equivalent to human hairs
- Bugscope Team Setae have many different shapes and patterns on them depending on their location and purpose
- Bugscope Team we 've noticed that they have a lot of spikey, spatulate setae

- Bugscope Team this, now, is the tip of the proboscis, where we see a lot of chemoreceptors
- Bugscope Team so it can sniff out your blood

- Bugscope Team this is one of its claws
- 7:21pm

- Bugscope Team this, now, is one of the most bizarre things of the day
- Bugscope Team you can see that we clicked on a preset but it is small and had shifted since we made it

- Bugscope Team letting you know that this is not 'canned'




- Bugscope Team sometimes we will help a school with high-mag focus, if we're near the 'scope
- Bugscope Team zooming out...
- Bugscope Team this is freaky
- Guest Is this the 3rd session we have had from Australia?
- Bugscope Team the claw we saw was stuck to the roach claw
- Bugscope Team Wow, we have the roach claw to the left holding onto a disembodied fly claw
- Guest Hi Lyn!
- Guest can we have control now? Lyn wants to drive
- Guest hi, im lyn from adelaide in south austraia. i work in em, and notice that your samples dont charge much - do you have any secrets in how you do that
Bugscope Team we make them bulletproof, coat with 20 nm of Au/Pd
Bugscope Team We also use a tilting, rotating holder in the sputter coater. 20nm is about 2-3x more than we normally use
- Bugscope Team Also, Lyn, we have enough samples on the stub so that we can avoid those that charge or that threw up on themselves when they died (and we hadn't noted that on the macro level).
- Guest good idea
- Bugscope Team We coat with a much thicker coat than you would use in research.

- Bugscope Team We use samples only once, as well. We don't want kids looking at someone else's samples.
- 7:26pm
- Guest Lyn, may be you can consider mailing bugs from down under and then have a session with a school in Adelaide.

- Bugscope Team Also worth noting is that we use double-sided carbon tape on the stub and even use a little dab of silver paint between the insect and the tape
- Bugscope Team if the school does not care to drive, we will drive for them; but once the kids know we can confer control to them, it's a free for all.
- Guest Lyn, here is snapshot of the stub, http://bugscope.beckman.uiuc.edu/overview.html
- Guest thanks this is great - i will suggest it at home
- Bugscope Team Umesh you know that Lyn has her own 'scope, doesn't need to work with us.
- Bugscope Team Great, we'll keep our eyes peeled for some Australian applications
- Guest i will hand over to someone else now bye
Bugscope Team Thank You, Lyn!

- Guest Yes, we will appreciate AUS applications. May be students training to be teachers could be interested.
- Bugscope Team we often find mold spores
- Bugscope Team sometimes pollen, sometimes bacteria, esp. on ticks
- Bugscope Team what is super interesting about ticks is that when their cuticle bursts, they just seal it back up agina
- 7:31pm
- Guest Here is the actual image, http://discord.itg.uiuc.edu/images/microscope/glennhand-300x204.jpg
- Guest Hello Bronwen!
- Guest bronwen here from brisbane hi. what machine are you using? I mean brand?
Bugscope Team Philips/FEI XL30 ESEM FEG
- Bugscope Team If you note the scale bar to the lower left of the image, 10 microns is about a tenth the width of a human hair
- Bugscope Team I think I've met you before. Now I'm a recluse and don't leave the lab.
- Bugscope Team So each segment of the mold spores here is about one hundred times thinner than a hair
- Guest Ah the old XL30 yes we have one too they're great for easy driving
Bugscope Team yeah Dude
- Bugscope Team the kids ask us if it's easy to use
- Guest We had it since Jan. 1999. Bugscope first session in March 1999.
- Bugscope Team this is a microscopy suite, so we have lots of equipment
- Guest By old I mean familiar - no disrespect!
Bugscope Team ain't no thang, DaddyO
Bugscope Team I was just recollecting how I've been using ours for 12 years.
- Bugscope Team once we had someone send owl pellets and the 'scope was down so we put them into the microCT and made a movie of the bones and skulls and teeth inside the pellet
- Guest Hi guys. I'm Chris from Brisbane. Are you both in the same room?
Bugscope Team it just happens, but Chas could be at home with his feet up
- Guest Hi Chris! I am in Washington DC. Scott and Chas are in Urbana-Champaign. Scott is driving. Chas is somewhere in town.
- Bugscope Team We were lucky to get support so that we could write our own custom software to control the microscope. That's been the foundation of Bugscope and is something we've continued to iterate on and improve ever since
- Bugscope Team Chris if you wanted you could join in to any session.
Bugscope Team On the main bugscope website their is a schedule with all of the upcoming sessions, all of which can be joined by the public
- 7:36pm
- Bugscope Team Annie used to connect in her pjs from her apartment. She was our entomologist extraordinaire.
- Guest haha
- Guest After 12+ years, it makes not much difference. Someone has to be in the suite.
- Guest Do you find it works better to be in the same room, or does it make no difference?
Bugscope Team what is funny is that we will write each other rather than talking
Bugscope Team For the people assisting the session? During the sample presets it's helpful to have a pair of people together but after that, for assisting on chat, it really is no different
Bugscope Team * while creating the sample presets
- Bugscope Team oh! also, we have a secret chat capability, so we can talk with each other over private chat on the same interface.
Bugscope Team Yes, that helps us ask each other questions without needing to confuse the other attendees
- Guest sneaky
- Guest Bugscope via smart phone, iPad, etc. Chas is already experimenting. I think it would be good to have other instruments form a network using software like Bugscope.

- Guest My hope has been a conference of Bugscope users in Urbana-Champaign. What works. What does not. and so on.



- Bugscope Team we're also going to upgrade our sister site, Virtual Microscope, which is ridiculously outdated.



- Bugscope Team one thing we will not ever do is videoconferencing
- Guest What are some of the future ambitions of bugscope?
Bugscope Team we're going to make yet another interface, and we're going to make *all* old sessions searchable; presently we go back only to 2006/7
Bugscope Team Our main goal the last few years has been improving experience of a participant, from before they apply through till their live session. Expanding to other instruments or other facilities would also be great, but it's not entirely simple; getting the right people running it is critical when working with K-12 groups


- 7:42pm


- Bugscope Team we want the kids to see the 'scope and what it can do, and drive if their teachers let them



- Guest Each classroom is like an investigator, with students and teachers. So, each session is unique. We have years of experience with unique sessions.



- Guest Thanks, Chas! I had been meaning to mention it. We should talk later.

- Bugscope Team this setup gives the kids and advantage, and they get it, they understand

- Guest Kate: Supreme court has a similar argument of not having video cameras. We just follow that :)
- Guest WE have to log off - this is Michele again! Take care and thank all of you!
- Bugscope Team also, this is another secret... we can look up things we don't know and answer them in near real time on chat
- Guest Thanks, Michele! Have a great rest of the day. Travel safely. Enjoy Australia.
- Bugscope Team Thank You!
- Guest Why no videoconferencing?
Bugscope Team think about how it would work... it would not give the kids a voice, and we would be, what, shouting into the microphone?
Bugscope Team One of the nice things about the text-based chat is that when more than one computer is available we can have kids asking questions in parallel, something that just wouldn't function with a single video feed
- Bugscope Team Thanks Umesh. Cya later, have a good night
- Guest Thanks Scott and Chas! Talk to you both later. Have a good evening.
Bugscope Team Thank You Umesh
- Bugscope Team Thanks Rachel!
- Bugscope Team And please tell Kate thank you as well.
- 7:47pm
- Bugscope Team over and out!