Connected on 2009-04-20 16:15:00 from , IL, US
- 3:48pm
- Guest thei am a guest trying t view session but i cant see anything i saw the microscope earlier now it just black.

- Bugscope Team can you hit refresh (F5)
- Bugscope Team see if that fixes it


- 3:54pm


- 3:59pm



- 4:05pm


- Bugscope Team hello, welcome to bugscope!
- Bugscope Team we are setting up presets for the session, we'll be ready by 4:15
- Teacher Hi. This is Dr. Clishem
- 4:10pm
- Bugscope Team hi dr. clishem, welcome to bugscope
- Teacher Are you running through the presets now?

- Bugscope Team yep, we are almost done


- Bugscope Team Hey what up Dr C?
- Teacher As you can see, a lot of my class logged in as "guest". Should they log out and log back in as "student"?
Bugscope Team no, guest is fine, no functional difference really
- Bugscope Team no either way is fine, really
- Bugscope Team we can confer control to whomever
- Bugscope Team ok, we are done with presets, you can now control the scope and we are ready to start the session
- Bugscope Team you wish
- 4:16pm
- Bugscope Team Dude we are ready to roll.
- Bugscope Team cool!
- Teacher Sorry, I was lecturing a bit. What a snooze.
- Bugscope Team no problemo... ;)
- Teacher I logged in as teacher. I don't see the presets. Wait. There they are. They just appeared!
- Bugscope Team magic

- Bugscope Team sometimes you may need to hit refresh (F5), that should always reload anything you might be missing



- Bugscope Team this is a digger wasp head, and body now

- Bugscope Team Tony's been doing this for a long time.


- Bugscope Team OOF
- Bugscope Team notice the scale bar in the bottom left of the image, that shows the size of what we are looking at. keep in mind that 1 um = one micron = one millionth of a meter
- Bugscope Team sorry had to focus

- Bugscope Team lotta juju on the eye
- Bugscope Team the compound eye is made up of hundreds, sometimes thousands of individual facets, called ommatidia
- Bugscope Team each ommatidia has a lens in it

- 4:21pm
- Bugscope Team those are the hexagon things
- Guest what is all the stuff on the eye
- Bugscope Team Caity there's a lot of dirt,mostly

- Bugscope Team this moth was caught in a trap so it is most likely stuff from other insects and dirt
- Bugscope Team some scales from other insects


- Bugscope Team i meant the ommatidia are squares. ommatidia = plural of ommatidium

- Bugscope Team sometimes we'll see mold spores, bacteria, brochosomes...
- Bugscope Team hmm, well they are still hexagons, but just barely.... wow, very cool

- Bugscope Team brochosomes are usually a few hundred nanometers in diameter, and they look like little soccerballs
- Bugscope Team so each ommatidium is about 11 microns = 11 millionths of a meter

- Bugscope Team brochosomes are produced only by leafhoppers, but you find them on other insects/arthropods they've, like, fraternized with.
- Bugscope Team this is the head of a digger wasp






- Bugscope Team the last four or five segments of the limbs, the distal segments, are called tarsi, or tarsomeres



- 4:26pm



- Student are we loooking at the stinger?
Bugscope Team not yet, that would be at the very end. I did look earlier for a stinger, but i didnt see one



- Student does the stinger break off when they sting?
Bugscope Team they do not break off for wasps. They do for bumblebees




- Guest what was that then?
- Student so what are we looking at?

- Bugscope Team stingers are modified ovipositors, and sometimes the stinger is also an ovipositor


- Bugscope Team here comes the end of the abdomen

- Student what is an ovipositor?

- Bugscope Team that was the narrow part of the body between the thorax and abdomen
- Student what is an ovipositor?
- Bugscope Team a digger wasp has a long, thin extended abdomen
- Bugscope Team ovipositors are egg-laying tubes
- Student so is this a female wasp?



- Student i assume that wasps are able to sting and not die?
Bugscope Team yes that's right. Actually I was wrong before, and it's the honeybee that dies after stinging, not the bumblebee. They die because a muscle attached to the stinger comes out as well that pumps and stinger further into its victim and also pumps more venom in the site

- Bugscope Team many parasitic wasps, which are often smaller, inject their prey with the wasp's eggs. for example if the prey is a caterpillar








- Guest The the callipillar have wasp babies?
- 4:31pm



- Bugscope Team yes wasps are able to sting repeatedly and live; bees leave their stinger in you, and it has a sort of outboard motor that continues to pump the toxin into your skin


- Bugscope Team yeah the caterpillars have wasp babies, but their insides are eaten by the babies -- so the caterpillars don'
- Bugscope Team t survive
- Guest What part is this?
- Student these look like hairs - are they?
Bugscope Team they are called setae (see-tee), and they help the insect to sense it's environement


- Guest What is the spikey thing sticking out?
- Bugscope Team the miscle attached to the stinger is what I was saying is kind of like an outboard motor
- Bugscope Team muscle
- Bugscope Team environment i mean
- Bugscope Team good job focusing Dr Clishem
- Student What other part will be good to look at?
- Bugscope Team Anshon we don't know what that is.
- Teacher Yeah, right, I'm a pro.
- Guest Is there any other part of the wasp that would be good to look at?
- Bugscope Team sometimes the antennae are nice to look at
- Bugscope Team those setae stick through the insect exoskeleton, to nerves underneath. some setae are mechanosensory and others can be chemosensory, so some of the hairs (setae) are used for smelling the environment!

- Bugscope Team or you can always go to another preset...
- Bugscope Team edge of the world

- Bugscope Team go south
- Teacher Let's go to Charlie Brown.
- Bugscope Team hmm, seems like you need to clikc on the image again to stop moving?

- 4:36pm

- Teacher Asleep at the wheel

- Bugscope Team the ant is fun because we don't usually see ants' heads look so round

- Bugscope Team Tony!
- Student why is this ant's head so round? or is that how they are supposed to look?
Bugscope Team we don;t know what kind of ant this is, but it is probably found like this among this particular species
- Bugscope Team dr. clishem, when using click to drive, make sure you click the image again to STOP moving. i think we are stuck in a moving state
- Bugscope Team click Done on the Driving thing
- Teacher I don't know what's happening!
- Bugscope Team okay I did that.


- Teacher Ah, you took away my car keys!
- Bugscope Team are you in click to drive mode?


- Teacher Maybe.


- Bugscope Team we are trying to fix the scope, whenever we set it to a preset it starts moving again, so i'm wondering if you are in click to drive mode and need to click on stop


- Bugscope Team ok, now we are good
- Bugscope Team you can try to click on a preset now
- Bugscope Team back to the extraordinarily round ant head

- Bugscope Team ah, this is the charlie brown ant head. so named because it's a circle head, like CB
- Bugscope Team yeah don't know what happened. aliens I think
- Guest What type of ant is this?


- Bugscope Team Anshon we don't know for sure.

- Bugscope Team you can see to either side of the antennae are small areas of compound eyes

- Student Why is it called Charlie Brown?
Bugscope Team well, i was being silly and called it that because it's a round head. young students sometimes like it when scientists fool around a little bit while learning. not that you all are young students...
- Bugscope Team we named it after one of the guys who works here


- Teacher Get rid of that click to drive mode.
- 4:41pm
- Bugscope Team this is the ball-and-socket joint at the antenna
- Bugscope Team the trick with click to drive is clicking once to drive and once to stop

- Bugscope Team but sometimes it seems to have its own agenda
- Guest are the antennae coming out of the nose?
- Bugscope Team a great alternative to moving around is to use click to center. i prefer that myself
- Teacher That's awfully kind of you to say that. I got a little too cocky.
- Guest What part of the ant is this?




- Student Is that the mouth?
Bugscope Team yep, with two things in front of it, chompers



- Bugscope Team the thing to the bottom of the image is paper
- Bugscope Team to the bottom you can see the paper strip this ant was attached to


- Bugscope Team for the mouth you can see it has a hinged jaw covering it
- Teacher Should we hand over the controls to someone else. Daddy's getting tired.
- Bugscope Team inside the hinged jaw would be palps (some are sticking out at the bottom) and a tongue
- Bugscope Team sure, who wants control?
- Bugscope Team we normally want the teacher to tell us who to give control to, then we'll do that
- Bugscope Team otherwise we'll have 10 kids asking for control all at once
- 4:47pm
- Teacher They are all very shy. Not like fifth graders.
- Bugscope Team it's impossible to break the scope, so please don't be shy
- Bugscope Team Violet has control now.
- Teacher Give the controls to Mari.

- Bugscope Team nice!
- Bugscope Team ok, mari has control now
- Teacher Violet is friends with Charlie Brown. She can have controls after Mari.
- Bugscope Team ok, just let us know when

- Bugscope Team mari don't be shy, ah, there ya go, nice!
- Bugscope Team this is an insect scale on the eye of a moth

- Student what are those things that look like leaves?
Bugscope Team those are scales of the moth
- Bugscope Team zooming out is a great way to get an idea of exactly what we are looking at
- Bugscope Team now you can see there are boatloads of scales,

- Bugscope Team see the tongue, all coiled up?



- Guest what should we look at?
- Guest what should be look at?
- Bugscope Team the minimum resolution is about 40X, so we can't go any less than that. the max is about 40,000x for bugs. although the scope can actually do up to 800,000x

- Bugscope Team Sana1 and Salma I think you should try another preset.
- Bugscope Team ask mari to click on a preset
- Bugscope Team find a preset that looks interesting, and then ask mari to click on it. #11 is wendy's salt

- Bugscope Team nice!
- Bugscope Team Moths are often difficult to image because of all the scales, which are not very good at channeling electrons away.
- Guest whose wendy?
Bugscope Team like the fast food restaurant
- Bugscope Team good job

- Student is this table salt? rock salt?
- Bugscope Team wendy's was dave's daughter, this is salt from the burger joint
- 4:52pm
- Bugscope Team wendy's salt, for some reason, it very cubic
- Bugscope Team we like this kind of salt because it has these sort of Aztec patterns in it.
- Bugscope Team it is not just like a boring cube

- Guest cool!
- Student wow
- Bugscope Team not a bug, really, but kids can ID with it well
- Bugscope Team the holes we think are due to another chemical -- an anticaking agent. but we really don't know what it is.
- Teacher Please let Violet drive now. Afterwards, maybe Peppermint Patty.
- Guest Give VIolet the controls please!
- Bugscope Team violet has control now
- Bugscope Team got 'em!
- Bugscope Team go ahead violet
- Bugscope Team Nisa I guess we could be spreading rumors, and we don't really know why the salt is like that. But it looks cool.
- Guest its going really slow
Bugscope Team hmm, sometimes network slowness can ause lag, what are you trying to do?
- Bugscope Team We like it that the salt has that appearance.
- Guest No more salt for me!
- Bugscope Team Violet it may be that the Internet connection is draggin'

- Guest what part of the lady bug is this?
- Bugscope Team um take the mag down like one click
- Guest What part of the lady bug is this?


- Bugscope Team if it's lagging up, stay away from "click to drive" and "focus". both those functions use live video, whereas all the others use single images so less bandwitdh
- 4:57pm
- Bugscope Team yeah okay where we wanted to be is above. inside the palp.
- Student whats the palp
- Student what kind of lady bug is this?

- Bugscope Team this is one of those Asian ladybeetles that bites, sometimes
- Student Do all bugs have Setae hairs?
Bugscope Team most do yes, it's interesting in that you don't really see those hairs with the naked eye. only when you put the bugs into a scope do you see these things.
- Student What is the palp?

- Bugscope Team palps are like little limbs around the mouth that are used to manipulate and also taste food

- Student what types of food do they eat?
Bugscope Team mostly plant matter

- Bugscope Team now we can see the tarsi, and you can see the pads on the tarsi with lots more setae -- tenent setae

- Guest where are the tarsi?
Bugscope Team tarsi are part of the leg segments, i think



- Student whats the wavy thing on the body
- Bugscope Team hmm, what wavy thing? i'm not sure?

- Teacher Please let Sana1 drive.
- 5:02pm
- Guest what is the background? the tiny dots
Bugscope Team those dots in the background are bubble in the double sided tape that the insects are adhered to
- Bugscope Team sana1 is in control!
- Bugscope Team watch the speed limit :)




- Bugscope Team cool, check out this claw, nice driving sana1!


- Bugscope Team now try focus, if focus + doesn't work, then try focus -
- Guest why is the claw shaped like that?
- Student Why is the claw shapped like that?
- Guest What is the "claw" used for?

- Bugscope Team well, it's shaped to help manipulate it's environment, and the claw can retract via the joint in the middle of it
- Bugscope Team the claw is used to grab onto things: food, leaves, etc



- Bugscope Team ah, these pad like things are called tenent setae

- Bugscope Team very cool little things they are: they help insects "stick" to walls and such


- 5:07pm

- Student What are we looking at now?
- Guest what is the spiracle all about?

- Bugscope Team this is a spiracle, used to breath, the air


- Guest what is a true bug?
- Guest Wha tis a true bug?
- Bugscope Team umm, i'm not sure, some kind of species, scott knows for sure
- Guest so the inside of the spiracle is shiny? or is it just light?
Bugscope Team that's probably just charging from the electrons inside the scope?


- Bugscope Team this is a hemipteran, a true bug


- Bugscope Team one of the things that makes true bugs true bugs is their piercing mouthparts



- Bugscope Team Actually Annie defended last week, has her PhD now
- Bugscope Team way cool annie!!!
- Guest what part of the true bug is this?
- Teacher Back to the bugs
- Bugscope Team I am sorry I had visitors here in my office
- Bugscope Team this looks like the tail end: the abdomen
- Bugscope Team this is the abdomen
- Bugscope Team you can see the folded wings
- 5:12pm

- Guest very cool! the spiracle is so tiny
- Bugscope Team take the mag down a little now
- Bugscope Team please



- Bugscope Team cool!
- Bugscope Team there's the pollen grain

- Bugscope Team to the left
- Bugscope Team way cool


- Bugscope Team is that a brochosome too?

- Bugscope Team wait, can't be, the res is too low for brochosomes
- Bugscope Team these are tarsomeres -- the tarsal segments of the leg



- Bugscope Team okay now if you use click to center t



- Bugscope Team you can d'oh




- Bugscope Team can I drive for a sec?




- Guest sure we want to see the first preset



- Guest thank u
- Bugscope Team there it is
- Bugscope Team don't sneeze!
- Student why are some people allergic to pollen, and some are not?
- Bugscope Team we could see bacteria if there were any
- Bugscope Team some people are just more sensitive, and there are lots of pollens

- Student thats okay, as long as i have benadryl, it doesn't matter!
- Bugscope Team heh
- 5:18pm
- Student thanks guys!
- Student thank you!
- Student Thank you for your time? Have a great night!
- Bugscope Team thank you!
- Guest thanks
- Guest bye. thanx. This was interesting!
- Bugscope Team dr. clishem, remember that your chat and images from this session are all saved to your member page: http://bugscope.beckman.uiuc.edu/members/2009-038
- Bugscope Team Thank You!
- Student thanks for a great class
- Bugscope Team you can visit that member page anytime to review the material from today, all chat and images are saved forever on that site
- Teacher Hey, real quick guys. My son is trying to decide between U of I and Lake Forest College for a degree in Communications. Any insights? Lake Forst is giving him $$$$$.
- Bugscope Team Thanks, Nisa

- Bugscope Team hmm, i don't know much about lake forest, but UI has a real good comm school, right scott?
- Bugscope Team Of course we like U of I.
- Bugscope Team Especially because once you get here you have a lot of choices -- you might find that communications is not really your thing.
- Guest do any of these bugs have a parasite?
- Bugscope Team linguistics is big here
- Bugscope Team Sana1 we did not find any parasites today
- Teacher OK. I figured. He likes U of I too. I think that will be his decision.. Thanks again. I will do the feedback as requested.
- Bugscope Team well, i don't know about parasites, but sometimes we find mites on the insects
- Bugscope Team very nice session today, well done!
- Bugscope Team wait, is a mite a parasite?
- Bugscope Team I think you'd have a better chance in the big world having gone to school here, compared to there.
- Guest i have always had this question... do any bugs HAVE parasites on them>
- Bugscope Team annie should be here... :(
- Bugscope Team like getting a job
- 5:23pm

- Bugscope Team yes, by definition a mite is a parasite, and i've seen many mites on many insects before



- Bugscope Team Sana1 mites are parasites in a way, and parasitic wasps as we mentioned earlier inject their eggs -- soon to be larvae -- into prey caterpillars


- Bugscope Team and the Varroa mite, for bees...

- Bugscope Team sometimes we'll see an insects with lots of mites on them...
- Guest do mosquitoes have a parasite?



- Bugscope Team we have not see parasites on mosquitos, but they could have gut parasites as well

- Guest would that be inside their bodies?


- Guest i am sorry what are facets again?
Bugscope Team facets are called ommatidia, a single one is named ommatidium


- Bugscope Team yeah the parasites would be inside the body, as parasites often are

- Bugscope Team each ommatidium has a lens in it

- Bugscope Team these are interesting because they have little bristles between the ommatidia
- Bugscope Team but the lens is fixed in one direction, unlike our eye which has a socket so the lens can move around
- 5:28pm
- Bugscope Team the bristles are mechanosensory -- they sense the wind speed
- Bugscope Team the wind direction
- Bugscope Team so what's better, a movable socket or a compound eye with 100's of fixed lens's arranged in a 180 degree area?
- Guest they have so much detail to them... its amazing, this creation
- Bugscope Team Roman where are you?
- Guest can you observe the cells through the electron scope?
- Bugscope Team we are seeing them now


- Bugscope Team those individual dots are much smaller than cells
- Bugscope Team we do have transmission electron microscopy as well -- TEM
- Guest oh really? i thought they were the cells

- Bugscope Team so we can see ultrathin sections of cells
- Guest right




- Bugscope Team those are usually 8 to 14 microns in diameter



- Bugscope Team bacteria -- the bacilli -- are usually about 2 microns long


- Guest i see
- Bugscope Team boy i learn something every day, i didn't know some cells can be as big as 8-14 microns.... interesting

- Bugscope Team those tiny dots were nanometers in diameter -- they were parts of cells
- Bugscope Team very cool
- 5:34pm
- Guest i thought the paper strip looked totally cool under the scopre as well
- Bugscope Team often we catch them manually and then freeze them, but many of the insects we looked at today came from a collection, and they were gassed in some way
- Bugscope Team yeah the paper -- cool to see the cellulose fibers

- Bugscope Team try http://bugscope.beckman.uiuc.edu/help for lots of info on how to do an entire bugscope session, from prep all the way to completion
- Bugscope Team I just drove us to the aphid head
- Bugscope Team you can see its eyes
- Bugscope Team on either side

- Bugscope Team and the piercing mouthparts


- Bugscope Team these were specially dried so they wouldn't shrivel

- Guest i see


- Bugscope Team you guys we are going to have to pack up and leave, let the next people get on the 'scope

- Bugscope Team we share the microscope with people doing their own research
- Bugscope Team the scope is used by researchers here at Beckman, for things other than looking at bugs
- Guest sure... thank you so much for this session
- Bugscope Team you are welcome!
- Bugscope Team but we have very much enoyed this session, and thank you Sana1
- Bugscope Team enjoyed...
- Guest ha.. sound kinda like annoyed
- 5:39pm
- Guest i am joking! this was great!
- Bugscope Team heh, well he certainly meant enjoyed, this was a good session, very good
- Guest i am sure the questions get redundant
- Guest thank you for all your help!
- Bugscope Team no problemo
- Bugscope Team ok, session locked, rxl disabled, session disabled, closing down now