Connected on 2008-12-08 12:30:00 from , PA, US
- 12:12pm
- Bugscope Team hi teacher! welcome to bugscope
- Bugscope Team welcome to bugscope!
- Bugscope Team Hello! Welcome to Bugscope!
- Bugscope Team are you able to see this chat?
- Bugscope Team let us know if you have any questions, or if you have any problems.
- Bugscope Team ah, welcome students! welcome to bugscope.
- 12:20pm
- Teacher Hi, this is Laura (Mrs. H-T) Thanks for having us!!
- Bugscope Team hi laura!
- Bugscope Team Good deal. You can see that 'teacher' has control of the 'scope.
- Bugscope Team And Alex has given you, Mrs. H-T control.
- Bugscope Team We can confer control to anyone who you would like to have it.
- 12:25pm
- Bugscope Team I can hear Alex, in the 'scope room, talking to you on the phone.
- Teacher I am going to get the kids to change their screens right now, so bear with us...
- Bugscope Team laura is going to change the students computers to 1024x768, and then we'll be ready to go!
- Bugscope Team okay, no problem laura, we are patient, just let us know when you are ready
- Teacher I'm back.
- Bugscope Team welcome to bugscope students from palisades!
- Bugscope Team Welcome to Bugscope!
- 12:31pm
- Student hi
- Bugscope Team Mrs H-T would you like to try driving the microscope?
- Student whats up
- Bugscope Team if you have any questions, please just ask, we will try to keep up with your questions
- Bugscope Team Hi Paul!
- Bugscope Team Hi Landon!
- Student hi
- Student Hi
- Bugscope Team Josh!
- Bugscope Team hi josh, everyone
- Student Hello! What are some of your favorite specimen to look at?
- Bugscope Team Nick -- can you all see the image on the screen alright?
- Student Can you put live specimens under the microscope?
- Student what
- Bugscope Team you are looking at live images from an electron microscope, in urbana illinois
- Teacher yes I would thanks
- Student what are the liines in the backround
Bugscope Team the background is carbon tape, which helps the insects stick and stay still
- Bugscope Team I especially like earwigs.
- Student why does the microscope have no light
Bugscope Team the microscope uses electrons to gather the image, NOT light, so that's why it's black and white
- Student Do you like this specimen and why?
- Student can you have more than one bug in the microscope?
- Bugscope Team The microscope is very complicated; it has thousands of parts.
- Student Is the Electron microscope named XL30 ESEM FEG
- Student Who invented this microscope?
Bugscope Team The first electron microscope prototype was built in 1931 by the German engineers Ernst Ruska and Max Knoll.
- Bugscope Team we don't image live bugs, although we could if they would hold still
- Student what are the little bubble like things
- Student what magnification is this
Bugscope Team the magnification is listed in the upper right, now it's 313x
- Student why is the micro scope so big
- Student what bug is that
- Bugscope Team this microscope is based on the transmission electron microscope, which was invented by Ernst Ruska in about 1931,
- Student Hi, I have a question, how do you vacuum out all the air of microscope?
Bugscope Team great question, there is a vacuum attacked to the scope, and it sucks out all the air when we pump it up
- Student what is the hardest part about preparing a slide?
- Student how much does the electron microscope weigh?
- Student how long does the electron microscope run??
- Bugscope Team the 'scope has to be big to hold all of the components.
- Bugscope Team It runs all of the time and probably weighs a ton or so.
- Student What kind of things do you have to do to bugs before you put them in the electron microscope
- Student what are the bubble like things near the top of the speciman
- Student cool
- Student how many people does it take to work the microscope?
- Bugscope Team mrs. h-t, go ahead and try clicking on a preset, that'll move the scope to that location.
- Student what bug is this?
- Bugscope Team the hardest part of making samples is getting the bugs on without breaking them or covering them with silver paint
- Student Can you show live specimens in electron microscopes?
- Student Is the name of the microscope called XL30 ESEM FEG
- Student Could you put a animal in the microscope?
Bugscope Team no, the chamber is small, the largest sample can be only 1.5 inches or so
- Bugscope Team this is a fruit fly, and you can see the spongy mouthparts
- Student how much can the electron microscope magnify?
- Student When did you get the electron microscope at your university?
Bugscope Team so we could look at really cool and interesting things close up, and learn more about them!
- 12:36pm
- Bugscope Team the chamber is small, relatively, and the stage has only 50 mm of travel
- Student why were there so many eyes
Bugscope Team that is a compound eye, many flies have compound eyes, it helps them to see well when they fly around
- Student can you show live bugs in the microscope?
- Student Is it possible to make an even smaller and or larger chamber?
- Student Is it hard for you to work this microscope?
- Student thats cool, do you guys look at anything other than bugs?
- Bugscope Team we can magnify over 600,000x but take useful images at no more than about 200,000x.
- Student Is it difficult to position the slide
- Student how many speciman have you worked with
- Student Sorry, I ment when not why,
- Student ?
- Student What is the highest magnification on the electron microscope?
- Student how big is the SEM
- Teacher I clicked on the tick, and it has not set up as of yet.
Bugscope Team ah! sorry, i forgot to unlock the session, try again now
- Student what is your favorite specimen you looked at?
- Student is it easy to preapare a slide?
- Student What kind of things do you have to do to bugs before you put them in the electron microscope?
- Bugscope Team we are trying to keep up with your questions, if we don't answer yours try again when it's not so busy
- Student Where else do you get specimans besides from schools?
- Bugscope Team Now try it!]


- Student How long does it take to prepare a slide?
- Student How can we control the stage?
- Student How much can the electron microscope magnify?
- Student how does themicroscope wprk
- Student How big is the electron microscope???????
- Bugscope Team there is a constant hum from the vacuum pumps
- Bugscope Team it's about as big as a large desk
- Student How long and tuff is it to get the job of working at the electron microscope

- Student what are the lines
- Student Could you put anything in it if it's small enough?
- Bugscope Team the samples are in a vacuum chamber, very dry, and we beam electrons at them
- Student why doesnt the electron microscppe ahve color?

- Student What is the most important part of the microscope?
- Student how do you use the vacum?
- Student When did you first get your electron microscope at the university?
- Student How long does it take the computer to process the electrons?
- Student whats your favorite specimen?????
- Student what are we looking at??
- Bugscope Team i think this scope was first here in 1998?

- Student Can you magnify anything as long as its small enough?
- Student what are those black holes
- Student what are we looking at
- Student how much does an electron microscope cost?
Bugscope Team this scope cost about $750,000 in 1998, right scott?
- Bugscope Team ah, this is a tick
- Student What is this specimen
- Student What kinds of things can you look at under the microscope, other then bugs?
Bugscope Team you can look at all sorts of things, metals, rocks, cells, bones, etc.
- Student how much did the mictropscope cost?
- Bugscope Team yay! the tick mouthparts
- Student what do you have to do to bugs before you put them in the electron microscope
- Student How long does it take the computer to process the electrons?
- Student how much does it cost today?
Bugscope Team all these images are live, so it works very very fast

- Student How strong are the electrons????
- Bugscope Team people look at plant parts, silicon samples, nanotubes, rocks, bacteria, all kinds of stuff
- Student What is this?

- Student How long does it take the computer to process the electrons?
- Student How do we get the image?
- Student WHAT bug is this
- Student what bug is this?
- Student I know that the electron microscopes take a lot of electricity. How do you manage to bring it in the building?
- Student sorry had caps lock on
- Student how many times magnified is this
- Student why does the microscope never get shut off
- Student why does the microscope never get shut off
- Student does any other microscope zoom in closer than the electron microscope
- Bugscope Team the microscope would be close to the same price now, I think.
- 12:41pm
- Student Have you guys ever look at anything like small chunks of rock, or parts of plants?
- Bugscope Team magnification is on the top right, 1479x right now
- Teacher My computer is not showing the image.
Bugscope Team try hitting F% refresh to get the image back
- Student how strong are the electrons?
- Student Are these the legs?
- Bugscope Team F5 i mean
- Bugscope Team a transmission electron microscope goes to a higher mag but in a different way
- Bugscope Team these are the tick's legs
- Bugscope Team you can see one of its claws
- Student is the microscope hard to operate
Bugscope Team no, once you learn it's not hard
- Student How long does it take the computer to process the electrons? I have heard that it takes a matter of seconds. Is this true?
Bugscope Team yes, these images are all live, so it takes a fraction of a second
- Student what is your favorite specimen that you've seen?
- Student ok
- Student can it fight or attack other bugs
- Student what are these
- Bugscope Team the electrons are coming at an acceleration voltage of 5000 V.
- Student what are the long pointy things?
- Student How much power does it take to power the microscope?
- Student how long does it take you to prepare a speciman?
- Bugscope Team those are setae -- the pointy things

- Bugscope Team hairlike sensory structures
- Student cool
- Student What kind of things do you have to do to the bugs before you put them in the electron microscope
- Student why are the microscopes so big
Bugscope Team well there are lots of associated parts to the scope, like a computer, electronics, mechanical parts, etc.
- Bugscope Team it takes 15 to 20 minutes to prepare a sample like this

- Student Why dose it look like armor?
- Student Is it IMPOSSIBLE to creat a electron microscope that shows the specimen in color?
Bugscope Team no, in fact, you can colorize the images based on elemental analysis after the image is taken, we've done that many times before

- Student What is the computer like that you use to run this microscope? It must have to be pretty fast
- Student create*
- Student is it hard to prepare a slide
- Student how much do the microscopes weigh?

- Student what is the biggest thing that can fit in this microscope
- Student What is your favorite specimen that you have seen in this scope?
- Student is this his mouth?????
- Student Is the electron microscope capible to view plants and/or solid objects
- Student Why is is it so hairy?
Bugscope Team those hairs are called setae (see-tee_ they help the insect to sense it's enviornment
- Bugscope Team the sample need only be air-dried, and we mount the insects on doublestick carbon tape with silver paint to help stick them down

- Student are thoose the teeth?
- Student Are those teeth??
- Student How long have you been doin' this?
- Student why are there so many crumb like things on it?
- Student what is your favorite specimen
- Student How much power does it take to power the microscope?
- Student Scot and Alex what kind of things do you have to do to the bugs before you put them in the electron microscope
Bugscope Team well, these bugs are coated with gold-palladium, which is what the electrons bounce into inside the scope, knock off, and those electrons are gathered and made into an image

- Bugscope Team there are electron microscopes that add false color to the images but we do not do that

- Student how long have humans used the electron microscope
- Student what was your first reaction when you saw this magnafied?
- Student what are those things inside it's mouth?

- Student What is argon gas used for?
- Bugscope Team Bryce we just have to stick them down and then coat them with gold-palladium using a sputter coater


- Student what is your favorite specimen that you have seen in the scope?
- Student What is gold-palladium?
- Student what are the hairy things?
- Student How many specimans have all of you worked with?
Bugscope Team many hundreds, if not thousands
- Bugscope Team argon is the insert gas we use that is excited into a plasma that attacks a gold-palladium target
- 12:46pm

- Student Q: those things in the mouth are they sharp to crush prey
- Student What do you use to run the microscope? I mean, did you have to make your own software?
Bugscope Team there is a computer connected to the scope, and it runs software made by the same company that built the scope. it's kind of cranky software, breaks a lot... :(
- Student what are those things in the spiders mouth
- Student what are the pointy things in his mouth
- Student If you put anymore of the argon gas on the specimen will it effect the image or specimen
- Bugscope Team gold-palladium is an alloy of the metals gold and palladium

- Student do you keep the sicroscope running all day every day?
- Student why does the spider have all of the hair in his mouth
- Student does the microscope run on a battery or just like an outlet?
- Student Are all electron microscopes the same size?
- Student Why is it blurry?
- Student Ok
- Student what is your favorite specimen you looked at?
- Student What are we looking at?

- Student how far can a electron microscope zoom in?

- Student what is this

- Student woww
- Bugscope Team it's blurry now because mrs. h-t is moving the scope, once she stops, it'll settle down and be clear


- Student How do you bring in that much electtricity into the building?
- Bugscope Team we can go over 800,000x but get good images at no more than about 200,000x
- Student O
- Student If u put any more argon on the specimen will it effect the image or specimen
- Student why is it so blurry
- Student what is the most interesting thing you observed


- Bugscope Team we have a high-tension tank that brings the voltage up in the room, behind the microscope
- Student how many specimens do you see in one day????
- Student are those the legs
- Student Do you like this job?
- Student what is your favorite specimen???????

- Bugscope Team the bubbles are craters in the carbon tape
- Student what is the coolest bug you ever seen in the microscope?
- Student Where do you get all the specimens for the microscope?
- Bugscope Team mrs. h-t, when driving, make sure to click to drive, and then you must click again to stop, or else it'll just keep going
- Student are those the legs???????????????
- Student alex what is your job
Bugscope Team i'm a systems administrator, work with computers, and help with bugscope too.
- Student About how many different specimens have your observed at your time in the university
- Student What is that!
- Bugscope Team I like earwigs best because they often have mites
- Student What are the holes?
- Student is your job fun or boring
- Student it had wings
- Student how many specimens do you look at in a day????
- Teacher okay, sorry
- Student whats the wierdest thing you have looked at under the microscope?
- Bugscope Team to the north is the fruit fly
- Student What is this?
- Bugscope Team not a problem mrs. h-t, you are doing great!

- Student About how many different specimens have your observed at your time in the university???
- Student cool
- Student what do you like most
- Student Scott and Alex,how long did it take you to learn how to operate the microscope?
Bugscope Team scott is an expert at electron microscope, he learned real fast
- Student what are we lokking at?????

- Bugscope Team we have seen some weird insects and some cool mites, not sure what is the coolest
- Student how many specimens do u prepare a day
- Student what is this
- Student ??
- Student why was there a tiny bug?
- Student huh
- Student how many specimens do you get to see a day??????????
- Student what are the leg things
- Student Alex, what part of your job do you love the most?

- Bugscope Team it takes a couple of months to get really good and know how to run all of the different modes
- Student Scot, how long have you been working with the electron microscope at the university?
- Student how many years of college do you have to go through?
- Student about how long does it take to prepare a specimen?
- Student how much do you work in one day (Alex)

- 12:51pm
- Student do you disect the bugs sometimes
- Bugscope Team we run bugscope three times a week, usually
- Student Do you think your job is fun? Would you consider us to do it?
Bugscope Team this job is a LOT of fun, and interesting, and it pays well too. you should definitely consider a job in the sciences, it's cool man...
- Student What are the holes?
- Student About how many different specimens have your observed in your time at the university

- Bugscope Team this is a super fun job
- Student how many specimens do you have to look at??????????
- Bugscope Team thousands of specimens over many years
- Student Scot, how long have you been working with the electron microscope at the university?
- Student Scott and Alex, After seeing a speciman several times do you get better at naming it's parts?
Bugscope Team YES! i used to know none of these things, now i know a lot
- Student Whats the biggest bug you've put in?
- Student what is that
- Student why don't you awser other peoples ?
Bugscope Team we are trying, keep asking if we miss yours!
- Student What is that?
- Student How much does it cost to run every day

- Student how many specimens do you look at a month???????????????????
Bugscope Team many hundreds
- Student what is the smallest bug you ever put in??
- Bugscope Team I have been working with electron microscopes for 25 years in university settings
- Student Scot and Alex. What's your favorite specimen that you have ever looked at?
Bugscope Team i like mites, they are little bugs that live on other bugs, a recursive bug!
- Bugscope Team Hannah sorry it is hard to catch all of the questions.
- Student have you made any interesting discoveries latey?
- Student Alex, I love science what field in science do you think would be the MOS fun
Bugscope Team i think astronomy is really cool, the starts, the universe, etc. wow!
Bugscope Team but you should do what YOU like, not what i like
- Student why is the microscope so big

- Student scot what is your job
- Student how did you know you wanted to do this job?

- Bugscope Team we work with guys who are making super hydrophobic surfaces, and that is pretty cool to see

- Student how small can the bug be to put in the electron microscope
- Student Alex, what do you enoy the most about working with the electron microscope?
- Student how small can the bug be to put in the electron microscope
- Student what are the holes
Bugscope Team those holes are bumps in the carbon tape
- Student Is this the moon?
- Student Why is it cracked?
Bugscope Team the inside of the scope is a dry place, so when it dries out, the crack form sometimes
- Student Is it hard for you to work with this microscope?
- Student how small can the bug be to put in the electron microscope
- Student what are we looking at???
- Student how small can the bug be to put in the electron microscope
Bugscope Team it can be very very small, but no bigger than an inch or so
- Student no joey it is not the moon
- Student what is this ?
- Student scot what is your job???
- Student how small can the bug be to put in the electron microscope
- Student why are there cracks
- Teacher my computer is slow to adjust my image, so I've been driving blindly
Bugscope Team try using "click to center" instead of "click to drive"
- Student are mites really really tiny??????????????????????
- Student cool
- Student how many parts are in the microscope?

- Bugscope Team Mrs H-T looks like you are doing well.
- Student Scot and Alex. whats the smallest thing you have put in the microscope?
- Student Alex, what do you enoy the most about working with the electron microscope?
- Student is that water
- Student what are the claw like things?
- Student Whats the biggest bug you've put in?
Bugscope Team one inch or slightly bigger
- Teacher But on my screen, I'm not seeing what my kids are, so that's what is difficult.
Bugscope Team not sure, want me to call you?
- Student how many little eye things are thers
- Student whay is the skinny thing


- Teacher good idea
- Bugscope Team we have looked at bacteria with 5-nm gold beads on their surfaces before
- Student Alex and Scot, when did you decide to go into science?
- Student i ment what
- Student i ment to put e

- Student is it easy to see a flys eyes
- 12:56pm

- Student can you put more than bugs under the microscope????????????
Bugscope Team yes! we put metals, rocks, bones, anything
- Student Alex, what do you enoy the most about working with the electron microscope?
- Student how many bugs do you have in your lab
- Student do you put plants under the microscope?
Bugscope Team yes, plants too
- Student does it have a nose

- Bugscope Team yes we can put more than bugs into the 'scope
- Student why does it have so many eyes?
Bugscope Team that is a compound eye, one whole eye with many little facets to it
- Bugscope Team they don't have noses - insects don'
- Student Does the hair protect the bug?
Bugscope Team not really protect, but rather it helps the bug to feel it's way around the world

- Student have u ever took the eyes out and observed them
- Student have u ever took the eyes out and observed them


- Student why does the eye ball look like a lot of bubbles
- Student What is the hair on the I's
- Student it looks weird
- Student Are the rocks very complex looking under the microscope?
- Bugscope Team we have broken the eyes before so someone could see how they are formed
- Student what are the hairy things on its head??????????
Bugscope Team those are called setae (see-tee), they help the bug to sense it's environment
- Student If you put rocks on it would you have to make it a certain size?
- Bugscope Team rocks are not always interesting
- Student can the fly see color
- Student after you look at the speciman what do you do with the data??????????????????
Bugscope Team scientists do all kinds of things, analyze it, study it, research it, etc...
- Bugscope Team yeah they would have to be small -- the rocks

- Student Oh.
- Student What iz below the mouth
- Bugscope Team the fly can see in color and may also be able to see UV light, which we cannot
- Student what do u do w/ the specimens after like do u draw what u see or....?

- Student Joy B. and I have disected a tadpole. have you ever looked at a baby tadpole?
- Student What is the most exotic thing that you have viewed under the electron microscope, Scot?
- Student why are thoes hooks on his foot for
- Student Have you ever observed money under the microscope?
- Student Joy B. and I have disected a tadpole. have you ever looked at a baby tadpole?
- Student what is the most big of thing you obzerbed
- Student why don't fruit flies have teeth
- Student have you guys ever made any discoveries with the help of the microscope lately??
- Student how come bugs are so hairy?
- Student does a fly have a hard protective body?


- Student Does the compound eye help the fly see better?
- Student what does the flipped h and p mean? 51 hp
- Bugscope Team we have looked at tadpoles in the past -- they need to be processed in order to look good
- Student how big can bugs be to look under the microscope??????
- Student Have you ever observed a sample of human skin?
- Bugscope Team Photis that is a Greek letter mu and an m
- Student kool
- Student how long did it take to process the tadpole?
- Student Scot, what is the best thing bout your job with the electron microscope?
- Student how long does it take to focus the speciman?
- Student descusting
- Bugscope Team it means micrometer, or micron
- Student why do bugs have hooks on their feet
- Student Where do you get the bugs?
- Student oh im greek. duh to me
- Student why aren't you answering my questions
- Student Scot, do you think some of the bug specimen's are disgusting?
- Student why does it's mouth look like a bee hive??
- Student how long does it take to focus the speciman?
- Student How much people work with you
- Bugscope Team Doug what I like is helping people get things into perfect focus
- Student alex and scot, how big can the bug be to look under the microscope????????
- Student what are the holes in the chin
- Student Does the compound eye help the fly see better?
- 1:01pm
- Student is it just you guys that answer questions?
Bugscope Team yes, right now, it's just us, we will try to answer everything we can
- Student ???
- Student Scot, do you think some of the bug specimen's are disgusting?
- Student what kind of bugs have you looked under the microscope?
- Student how many parts are on that bug
- Student why is the fly so small
- Student how long does it take to focus the speciman?
Bugscope Team oh, just a few minutes. focus can be tricky though
- Student Does the compound eye help the fly see better?
- Student ogh
- Bugscope Team Catherine some of the bug specimens are pretty gross, but we are used to it
- Student What is your favorite part of working with the microscope?
- Student What are we looking at?
- Student Have you ever looked at a small fish?
- Student Alex, how long have you been working with the electron microscope?
- Student How come bugs are so hairy?
- Student so, you look at anything bigger then a cicada?????????????
- Student Is it?
- Student why is the microscope so big
- Student do you have any of these specimans as pets
- Student Does the compound eye help the fly see better?
- Bugscope Team Michael the compound eye produces lots of tiny images that we think are composed into a fuller large image in the brain
- Student Do you have someone tell you what to look at in the lab?
- Student Is it tough having 2 people answering to 25 kids?
Bugscope Team well, we try
- Student is it hard for you to work the scope?
- Student scot, you cant look at anything bigger than a cicada?????????????
- Student why does the mouth have holes
- Student can you look at anything from like the ocean or lakes
- Student have you looked at any thing other than bugs
Bugscope Team yes, lot
- Guest hi
- Student What is the hardest thing to do with the electron microscope, Scot?

- Student is this still the fruit fly?
- Guest hi any one
- Student cool
- Student is your job hard
- Student ???
- Student is it hard for you to work the scope?????????????????

- Student hi mo
- Student hi moe
- Bugscope Team Doug sometimes the hardest thing is working in wet mode, which we are not doing today. It is tricky to run the 'scope that way.
- Student what are we looking at????????
- Student have you ever disected anything and put it in the microscope

- Student hi mo
- Student Cool
- Bugscope Team Wyhen we work in wet mode we do not need to make the sample conductive.
- Student what are we looking at
- Student what are the lines on the head?
- Guest my name is relly molly
- Student what is the most imortant part of the microscope
- Bugscope Team This is the head of an ant
- Student where do you get the speceimens???

- Bugscope Team Hi Molly>
- Student what are the line things in the front
- Student is it hard fo ryou to work the scope???????????
- Student what are thoes lines on his head
- Guest sup
- Student can you alive specimens on?

- Student What is at the bottom of our screen?
- Student why are the pictures black and white
Bugscope Team the scope uses electrons to gather the image, not light, and since color is gathered from frequency of light, and since there is no light, it's just black and white, or intensity of the electrons
- Student can u see any food paticles or molicules after argon is ammited
- 1:06pm
- Bugscope Team the lines on the head are how the cuticle is shaped
- Student what are the lines
- Student what is the biggest part of the microscope
- Student Have you every looked at cells? If so, is it easy to ee the cell's parts?
- Student can you look at alive specimens??
- Student see********8
- Student mo your causing trouble
- Student on its back
- Student stop talking to us mo
- Guest oh no
- Student is it hard for you to work the scope????????????????
Bugscope Team no, it takes some getting used to, but it's not hard once you learn, like anything else
- Student oh cool what does that do
- Bugscope Team we can see food particles but usualy we do not see small molecules
- Student Ahh


- Student Do most specimans you observe have hair that can not be seen by the naked eye?
- Student how many schools do you help in one day
- Student ewwwwww
- Student Have you ever seen an individual cell?
- Student why are bugs so hairy
- Student has the micro scope ever broke? did you have to pospone your site?
- Student Do you kill the specimens or find them... dead?
- Student what is the smallest speciman you have ever worked with?


- Student how far can the electron microscope zoom in???
- Student And if so did you see the nucleus?
- Student how does it get so close without crushing the bug
- Student Has the micro scope ever broke? Did you have to pospone your site?
- Bugscope Team Emily we often see fine hairs (setae) that may not be visible simply by eye
- Student Do you kill the specimens or find them... dead?
Bugscope Team well, the specimens need to be stable, so dead is really better, we often freeze them to kill em, it's more humane that way?
- Guest i eatbugs
- Student do you work every day
- Student Do you kill the specimens or find them... dead?
- Student What are those white things?
- Guest me
- Student is there only one microscope at the university?
Bugscope Team no, there are several, we have 5-7 in our lab alone

- Student ok
- Bugscope Team sometimes Carolyn we kill bugs, mostly by freezing them
- Student why are bugs so hairy
- Student Ahh got'cha
- Student how many microscopes do you have at the university?

- Student do you have more than one electron microscope?
Bugscope Team yes, we have two, transmission and a scanning

- Student Is there only one type of electron microcope?

- Student Has the computer running the microscope ever crashed
- Student Won't freezing them just preserve them, like Walt Disney?
Bugscope Team ha!, well, i think in most cases it kills them, although i think once a bug came back to live aftetr being frozen, scott might remember?
- Student how much do you use the microscopes
- Student What are those white things?
- Bugscope Team Mueller the hairs (setae) often have sensory functions -- they allow the insect to sense its surroundings
- Student how does the scope magnify?????????????????
- Student it looks like a face
- Student what is the oval thing on it's head?

- Student does the ant have a protective layer
- Student how far can the electron microscope zoom in???
- Student Can you look at liquid under the microscope?
- Student so why do they need so many of them
- Student can you look at water under the microscope?
- Student What is the vacuum pressure in the chamber when its being used?
- Student jow much wieght can 1 ant lift
- Student how much energy does the electron microscope use?

- Student what are the lines on its back
- Student how does the scope magnify??????????????????????
- Guest stop talking abot bugs
- Student Can you look at liquid under the microscope?
- 1:12pm
- Guest its gross

- Student Oh well
- Student what is that black dotin the upper left hand corner
- Student Has the computer running the microscope ever crash
Bugscope Team yes, occasionally it does crash, and then we fix it
- Student what are the cracks in the background??

- Student is that dust on the ant
- Student how does the scope magnify????????????????????????????
- Bugscope Team Kristen the 'scope magnifies by putting a beam of electrons on a small area, and the electrons cause 'secondary electrons' to be ejected from the sample. We can collect the signal from the secondary electrons
- Student Why does the wasp have big eyes, and a big antenna


- Student why does the microscope have to run all day
- Student why are the indents in the eyes???????
Bugscope Team those are facets of the compound eye, called ommatidia, they are like little individual eyes, each with a lens in it
- Student Scot, what is the vacuum pressure in the chamber when its being used?


- Student how long does it take to start the computer back up if it crashes?
- Bugscope Team Catherine you can look at liquid when you use wet mode.
- Student why are there hairs on the eyes?
- Student is it hard to use the electron microscope
Bugscope Team no, you get the hang of it after a while, like most things in life


- Student what does the wet mode do?
- Student Whats the bump?
- Student are there hairs on the eyes
- Student Have you ever looked at the beam as it was working? or does it blind you?
- Student What's in the backround?
- Student Alex, what is the vacuum pressure in the chamber when its being used?
- Bugscope Team Doug the vacuum is better than 1.3 x 10-4 mBar now. It is hard to translate those numbers into something meaningful.
- Student why are there little hairs on the eyes?
- Student Oh ok
- Student what measurement is backwords "u" m?
- Teacher Yup, I'm getting there
- Student Can you look at gases under the electron microscope?
- Guest HI KAT
- Student Have you ever looked at the beam as it was working? or does it blind you?
- Student are the eyes bumpy
- Student mBar = millibars?
- Student let me rephrase that, how do you use the wet mode?
- Student Has anything dangerous ever happened with the microscope in operation?
- Student how come the microscope has to run 24 hours
- Guest hi mo
- Bugscope Team um = micron = one millionth of a meter
- Student are thoes pointy things hairs
- Student Have you ever looked at the beam as it was working? or does it blind you?

- Guest sup
- Bugscope Team Savannah the um is a Greek letter 'mu' and an m. It means micrometer -- one millionth of a meter.'
- Guest nothing
- Student how long do you freeze the specimans?
- Student what is the long stick thing
- Guest how old are you


- Student Why do you want to look at bugs for a job. Is it important? Can you help the world with it
- Guest im nine
- Student how much energy does the electron microscope use??


- Bugscope Team Photis the beam does not work when the microscope chamber is not under vacuum, so the beam cannot get out.
- Student Scot, when you said "mBar" mean "millibar?"
- Student Have you ever looked at the beam as it was working? or does it blind you?
- Student why does the 'scope run all day long?
- Student How do you make money?


- Student oh ok. sorry for asking twice
- Guest or girls
- Bugscope Team This is part of what we do.
- Student wooowwww
- 1:17pm
- Student why is there so much hair
- Bugscope Team Doug yes 'millibar.'

- Student cant be bigger than an inch. Right Scot?
- Guest there hairy like my dad
- Student ok
- Student ok
- Student how do you use the wet mode?
- Student i never thought of a measurement smaller than a millameter, Scot.
- Student Ok, thanks Scot
- Student is that a mosquito
- Student What are we looking at again?
- Student WHAT is wet mode
- Student is that a big nose
- Student Has anything dangerous ever happened with the microscope in operation?
Bugscope Team not that i know of, although there is liquid nitrogen that is put into the scope sometimes, and that scares me!!!
- Bugscope Team Brandon the hair is used because the insect needs to be able to feel the environment from behind its shell, which is like armor.


- Student what are the lines winding into eachother?
- Student it looks like legs coming out of its mouth
- Student Wet mode is where u can c water.
- Student has the electron microscope ever broke down?????????????
- Student why are the insects bodies look like they have plates on them
- Student What are we looking at?
- Student what do you look at the most


- Student Ohh well i guess it's good no one has ever gotten hurt
- Student Liquid nitrogen? What is that used for in the microscope?
- Student what are the lines winding into eachother?

- Student What is the liquid nytrogyn for?

- Student Thats long.
- Bugscope Team Paul we look at carbon nanotubes, coral, silicon structures, grass, seeds, bat embryos -- you name it.
- Student has the microscope ever broke down??????????????????
Bugscope Team yes, it breaks sometimes, and we have to fix, or call the company to fix
- Student What kind of other objects do u look at?
- Student why is it so bumpy and hairy

- Student that is cool
- Student What are we looking at?
- Student is that long thing the mouth or nose
- Student this true bug is cool
- Student Have you ever looked at coins?
- Student are these the legs???????
- Student Has any specimen ever made your laugh? If so, which specimen (if you guys can remember)

- Student what are the things that look like cob webs?
- Student If we looked at our face with a microscope, would it be that hairy?
- Student have you ever put anything else in the scope, other than the other things you mentioned?

- Student what are thoes hais on its legs
- Student what are the things that look like cob webs?
- Student ??how much volts does the electron microscope use??
- Student How long does it take to fix?
- Bugscope Team Joey we had a Monarch chrysalis in here and the butterfly hatched later.
- Student why does it look like there is water on the bug
- Student Liquid nitrogen? What is that used for in the microscope?
- Student Is the flat part where thay put the food to eat it?
- Student are these thr legs?????????
- Student what is a carbon nanotubes???
- Student oh
- Student Have you looked at dollar bills?
- Student How long does it take to fix? The microscope that is.
- Student are these the legs???????????
- Student Is the flat part where thay put the food to eat it? Is is called the foodimg station? sorry but i think its that
- 1:22pm
- Bugscope Team The liquid nitrogen is used to cool a field-effect transistor in the x-ray analysis system.
- Student Oh alright
- Student what is the line coming down the stick thing
- Student how many times has the microscope broken down
Bugscope Team oh, i can think of 5-10 times in the last 8 years maybe?
- Student what is your favorite specimen scot
- Bugscope Team we have looked at Mexican paper money before.
- Student was that long thing the mouth or nose
- Student How long does it take to fix? The microscope that is.
Bugscope Team we try to fix it ASAP, beacause researchers are trying to use it to get their work done
- Student have you ever looked at coins?

- Student What is the worst thing that has happened when working with the electron microscope?
- Student have you guys ever made any huge discoveries lately??
- Student does the scope break down a lot
- Bugscope Team Josh I like mites, so often we look at earwigs to see if there are any on them.
- Student Have the bugs ever bitten you as you tried to kill it


- Student why does the bug have a gotee
- Student it looks like a beard

- Student do you like this specimen?????????
- Bugscope Team We have looked at coins, and we have had bugs try to bite us.
- Student X-ray analysis!?
- Teacher This bug looks like Mr. Hinkle!!
- Student What is your favorite specimen you ever looked at? And why is it your favorite?
Bugscope Team i like mites, because they are bugs on bugs, recursive bugs!
- Student cool me two

- Student oh
- Student hahahhaahaha
- Student That is MEAN!
- Student Are you guys seeing exactly what we are right NOW?
Bugscope Team yes, we see the same thing you do
- Student hahaaha1
- Student cooooooollll
- Student Paul, don't ask wierd questions
- Student Uh-oh what?

- Bugscope Team Yes Catherine.
- Student awesome
- Student Mrs. H-T, your funny!!!
- Student Looks like our teacher mr huin
- Student what are those hair things?
- Student what is thet?????
- Student What is the two things comming out of the hole?
- Student Thanks
- Student sorry typo
- Student there are these two lines coming out

- Student YEaaa!
- Student yup
- Student WHAT IS THAT????


- Student yep
- Bugscope Team Joey I was thinking Mr Hinkle must have a good sense of humor
- Student what are thoes two things coming out of his mouth?????????????
- Student is that dust?
- Student is that food stuck in the mouth?
- Student What is the gunk on the bug
- Student are most mites big or small
- Student what are the crum thing
- Student na
- Student Nope
- Student what is that dust stuff?
- Student no mr hinkle doesnt he always makes fun of me
- Teacher Yes he does, even though his jokes aren't that funny!
- Bugscope Team sometimes i see myself in a bug too, you know, if the bug is like really handsome and all
- Student is that dirt?
Bugscope Team yes, some of this is dirt sam, or just grime, if we aren't sure what to call it, we call it juju
- Student exactly
- Student hahahahahaha
- Student What are those two things?
- Student Why does that look like dust
- Student what is coming out of the bug....a mouth?
- Student what are those carrot looking things
- Student i see one of my friends like a car
- Student what are the two pointy things sticking out????????
- Bugscope Team we don't know just what those are
- Student what is that?!?!?!?!?!?!?!!?

- Student
- Student what is that?
- Student the things coming out of hole, are those the mouth???


- Student
- Student
- Student juju..... ok... oddd. what does juju mean?
Bugscope Team juju means it's some kind of stuff, maybe dirt, but we aren't sure, so we call it juju
- Student Woahhhh!
- Student what is the dust stufff
- 1:27pm

- Student How do you have a computer out here in Pennsylvania control the electron microscope out in Illinois? (sorry if I spelled that wrong) I know you can remotly control stuff, but still, that's a long distance.
Bugscope Team all this is done over the internet. the computers and the scope are in urbana, illinois, and you are controlling that scope live through the internet. nothing else is inbetween us and you
- Student

- Bugscope Team mueller it is prolly dust or dirt like you said: dust stuff
- Student oh. could it be food? lard?
- Student

- Student what are the 2 sticky things?!?????????
- Student cool
- Student Has anything you have looked at made you laugh?
Bugscope Team we thought the mr. hinkle bug was pretty funny!!!!
- Student hi cate!!!!
- Bugscope Team There is lots of dust on these samples because of the way they were processed.
- Student Hi cate!


- Bugscope Team hi all!

- Student Oh ok
- Student Has a specimen ever freaked you out?
Bugscope Team we once got specimens from one school, and scott opened up one of the packets containing some insects and he screamed! there was a giant millipede in it that looked like a mini snake. It was pretty gross looking
- Student hi
- Student oh, so we were looking at the tick mouth all this time?!?!
- Student This is a mouth?
- Student is it easier to use a light or a electron microscope
- Student is that a tick
- Student cate, what is your favorite specimen????????
Bugscope Team bees are cool, they have stingers!
- Student Hahahahahahah!
- Bugscope Team This is the part of the tick that pokes into you, called the capitulum.
- Student HaHaHa!
- Student it looks excatly like his beard or gotee
- Student does it have teeth (tick)

- Student what is the funniest thing you experinced in the lab cate

- Student eww
- Student why are there holes
- Student what are those hole things?
- Student Why is it called a yellow wing?
- Student what is that
- Student Has a specimen ever freaked any of you out?
- Student can you show us a stinger???
- Student What is this??
- Student what is with the lines
- Student cate, do you like this specimen????
- Student what are the stripes
- Teacher I know it's almost time for us to go, but I would like to pull out on this wing first if that's okay.
- Student have you guys ever made any discoveries lately??????

- Student Cate are you new to this
Bugscope Team ive been working with bugscope for around 2 1/2 years
- Student Have you ever looked at an inanimate object under the microscope?
- Student what are the strips
- Student What is the size of an electron?
- Bugscope Team mrs. h-t, you are doing great! this is a scale, part of a wing, but it's very very small
- Student why does it look like a lot of holes
- Student it looks like the coliseum on its side


- Teacher Thanks for being fo nice to me...
- Student how fun is your job
- Teacher How about so
- Student Bye Bye Admins


- Student just in case i couldnt
- Student have you guys ever made huge discoveries lately

- Student Cate, are you a teacher or a student?
Bugscope Team im neither, I just work full time as a scientist!
- Student Why is it called a yellow wing?
- Student Did you ever get freaked out by a bug under the scope?
- Bugscope Team mrs. h-t, all the chat and images from today's session are stored on your member page: http://bugscope.beckman.uiuc.edu/members/2008-109

- Student scot, do you like this specimen????
- Student What college did you graduate from cate?
- Bugscope Team that way you can look at all the chat and images afterwords, study it and such
- Bugscope Team Savannah nothing huge, just small steps.
- Student scot do you work on this job a lot
- Student what is the MOST digusting thing you have ever looked at?
- Bugscope Team Yeah Sasha I like it.
- Teacher I can't wait to see all the questions/answers I've missed.
- Student what are the pointy things
- Student Cate, what is the coolest thing you've done/seen with an electron microscope?
Bugscope Team ive seen a lot, it's kind of hard to pinpoint just one thing. Ive seen some pretty cool e.coli bacteria using the microscope you are using
- 1:32pm

- Student thanks
- Student do you like your job cate?
- Student That kind of looks like plates. What are they?
- Bugscope Team see how those scales make up the fur on a moth, cool huh?
- Student alex why does it look like a bunch of duck feet
Bugscope Team heh, well, things look like other things sometimes, but these are scales on a moth
- Student Fun

- Student scott how long have you been working with bugscope?
- Bugscope Team Mrs H-T we are afraid to look at what we've missed.
- Student do like the job cate
Bugscope Team yes i do, it is fun
- Student tytypo, sorry
- Student what are we looking at
- Student Oh that's cool
- Student Cate how long have you worked on the electron microscope?
Bugscope Team i have been working with it for around 3 years

- Bugscope Team Shelby since it started almost 10 years ago.
- Student what is that????
- Student these look like scales \
- Student are those fins cate
Bugscope Team those are individual scales on a butterfly wing
- Student What do you do at your job cate
Bugscope Team i help users with their projects related to electron microscopy and i do what im doing now-- help run bugscope

- Student It looks like duck feet
- Student what is this??
- Student looks like a bunch of duck feet
- Student what is that dust at the bottom
- Student sorry, i never ment to type the slash
- Student what is it cate
- Student What job would you recommend to a student? In science, that is.
Bugscope Team find something you like: fish, stars, small stuff, huge tractors, and then start reading about that stuff, and then go to college and learn more about it! but it all starts with you finding what you like
- Bugscope Team Savannah those *are* scales; you are right.

- Student alex how long have you worked here????
- Student is that dust near the bottom
- Student what is the most digusting thing you have ever looked at
- Student why are there so much fin-like things
- Student Alex, why are the scales shining?
- Student what is this image or bug. our science teacher is going CRAZY
- Student what type of butterfly cate
Bugscope Team some sort of yellow butterfly, i dont know what kind sorry
- Student the white part looks like europe
- Student alex how many people work with you
Bugscope Team right now, there are 3 working on bugscope, we have about 20 people in our group though
- Student are thoses scales??????
- Teacher I hate to end this, but our kids have to get ready to go home...thank you SOO much for having us today! This was so much fun!
- Student alex how long have you been working with bugscope?
- Bugscope Team Thank you!
- Teacher Okay all you questioners, you must log off now... say goodbye.
- Student what is that
- Student why are there so many scales scot?
- Student no scratch that it looks like hawaiii
- Bugscope Team thank you for all your great questions, sorry I didn't get to see more
- Student bye bye cate!
- Student thanks!
- Bugscope Team Yes those are scales, Savannah!
- Student by
- Student BYE!!!! and thanks
- Student bye thankls admin
- Student Byyyyyyyyyeeeeeeeeeeeeeeeeeeeeee!!!
- Student thanks! bye
- Student thank you
- Student Byezorz
- Student bye
- Student goodbye, thank you!!!
- Student Bye Admins Cate Scott Alex
- Student Thank you!
- Bugscope Team Thank you!
- Bugscope Team Bye!
- Teacher I am having the kids fill out a 6 question survey, which I will send to you...I'm sure they will have some real interesting answers
- Bugscope Team Awesome
- Teacher The question scroll on the right is a great feature.
Bugscope Team thanks! it helps to pick out the really imporant question/answers
- 1:37pm
- Bugscope Team okay, thanks laura, great session, remember all the chat and images are saved to: http://bugscope.beckman.uiuc.edu/members/2008-109
- Teacher Thanks! Talk to you on Wed (next session)
- Bugscope Team ciao!
- Bugscope Team we look forward to it!
- Bugscope Team See you Wednesday.
- Bugscope Team It is the wing of a large moth.
- Teacher got it..bye!
- Bugscope Team alright, closing down the session