Connected on 2008-05-28 13:15:00 from Park Ridge, IL, US
- 1:16pm
- Teacher hello we are here now
- Bugscope Team yay!
- Bugscope Team hello!
- Bugscope Team welcome to bugscope!
- Bugscope Team you should have control of the scope now, you are welcome to start driging it anywhere
- Bugscope Team Hi Rick!
- Bugscope Team driving it, i mean
- Bugscope Team Oh Jimmy-John's is on!
- Bugscope Team and Julie and Grace!






- Bugscope Team this is the face of a cricket, of course

- Bugscope Team feel free to ask any questions, we are here to guide you
- Student what is the most interesting thing u have ever seen under a microscope
Bugscope Team well bugs are very interesting. there are lots of neat things on bugs that you can't see with your eyes: spiracles, setae, spinnerets, etc...
- Bugscope Team if you drive a little south you can see the mouthparts



- Bugscope Team of bug-like things I like finding mites on insects
- Bugscope Team the jumping bush cricket is the most common type of cricket in the US
- Student did you always want 2 be a scientist
Bugscope Team scott and cate are scientists, i'm a computer dude
Bugscope Team I've always liked science, working with microscopes makes it so I always get to be around it
- Student how do they chew
Bugscope Team they should have some type of jaws, but you can't see them easily here
- Bugscope Team I like seeing stingers on bugs, they are cool
- Bugscope Team I was in danger of being an English teacher and decided I'd better take some biology classes so I could do something else


- 1:22pm
- Bugscope Team so I got a degree in English and Biology and have been doing this (electron microscopy) for a long time
- Student y does it look like it has hair on their eye
Bugscope Team that hair is called setae (see-tee), it is connected to nerves underneath the exoskeleton. those setae are how bugs sense their environment
- Bugscope Team they often do have what looks like hair
- Student y
- Bugscope Team the hair-like things are called setae
- Student what do they do
Bugscope Team the setae (hairs) have many different functions on insects. in general, they help the insects sense what is around them. Some will taste/smell/feel
- Bugscope Team and setae are often sensory -- they can help the insect feel its environment, and/or they can help it taste the air, or smell
- Student what do you do at your job???
Bugscope Team We do this...I also train people on the microscope you are using now as well as another electron microscope- the transmission electron microscope (TEM)
- Student Why did you choose this job?
Bugscope Team well, a job in the sciences and technical field is very interesting, you get to do different things each day, and it makes you feel good to use your mind

- Student HOW WELL IS YOUR VISION

- Bugscope Team we train people to use microscopes, of all types, so they can use them to do their own research



- Student what is the long tube going down the stink bug's head
- Student What are we looking at?
- Student how do they eat
- Bugscope Team mostly we work with graduate students, sometimes undergraduates, and often postdocs -- people who have their PhDs and are just doing research
- Student what are we looking at
- Bugscope Team the long tube is part of the proboscis

- Teacher what does this part do
- Bugscope Team stink bugs are true bugs, which means, for one thing, that they have piercing mouthparts
- Bugscope Team this is part of the mechanism that lets the stinkbug move its proboscis around
- Student why are there rippals on the proboscis

- Bugscope Team with a proboscis, you can think of it as an elephant's trunk, it can suck up juices through it
- 1:27pm
- Student Whats the pointy things on it
Bugscope Team those are more setae (hairs), and their function is to sense the wind movement. So if you swatted your hand at it, the fruit fly could feel it coming
Bugscope Team those are setae, sticking out inbetween the facets of the eye
- Student what are we looking at now???

- Bugscope Team it is either muscular or like one of those party favors that extends when you blow into it
- Bugscope Team this now is a fruit fly compound eye
- Student are bugs warm blooded or cold blooded
- Bugscope Team they are cold-blooded, so they move slowly when it is cold

- Student what is this?
Bugscope Team monarch butterfly scales from a wing


- Bugscope Team this is part of a butterfly's wing -- the scales
- Student thank you
- Bugscope Team these are tiny scales from a monarch butterfly wing
- Student is this a cell
- Student do you know the average of how fast the bugs you look at go??
- Bugscope Team the scales are kind of like feathers to a bird
- Student why are there lines on it
- Bugscope Team notice the holes in the scales. those holes allow for lighter scales, yet the wing still maintains good lift in the air for flying

- Bugscope Team the lines are ridges that help it keep its shape, and they are also thin enough that they refract light in different colors


- Bugscope Team OOF as scott would say (out of focus)
- Student do u no how the tear happend?
Bugscope Team that could happen if someone were to touch the wing. It can tear easily, so I don't know the cause of it, maybe it happened when I picked it up
- Bugscope Team we think the scales are used to help give the insect lift in the air, but they also serve to help keep the insect from getting stuck in webs
- Student what are the lines on the wing
- Bugscope Team if the butterfly runs into a web the scales will stick and fall out and he/she can leave
- Student What is best thing that you have ever looked at?

- Teacher what is a palp
Bugscope Team it helps the insect to taste and manipulate food
- Bugscope Team this vacuum nozzle is a ladybug palp
- 1:32pm
- Bugscope Team we did not answer the question about the average speed of these insects in part because it is hard to be definite about that
- Student is this basically it's mouth??
Bugscope Team no, it has 2 of these big palps on either side of its mouth

- Bugscope Team the palps are mouthparts that resemble accessory limbs
- Student are those spikes or hair
Bugscope Team they are hair
- Bugscope Team this is right next to the mouth -- we won't be able to see an open mouth on this ladybug
- Student how does the bug contain water in its body
- Student what is in the inside of the vacuum?
- Student what are all the dots in it??
- Student are they its tastebuds??
Bugscope Team they are analogous to tastebuds for us, yes!
- Bugscope Team those are sort of like taste buds, inside the vacuum cleaner nozzle -- yes
- Bugscope Team D'Oh!


- Bugscope Team Cate is too fast for me
- Student what are the little dots


- Student what are the bumps under the mouth

- Student DO THEY HAVE A TOUNGH
- Bugscope Team the little dots were the individual sensory buds
- Bugscope Team ladybugs do not have a tongue
- Student are we seeing scales or cells
- Student what may this particle be?
- Student what is that?
- Bugscope Team they have chewing mouthparts
- Bugscope Team it is juju we do not recognize
- Bugscope Team may be oil from the sputter coater
- Bugscope Team like, a droplet of oil

- Bugscope Team here is one of the claws


- Student can u tell us about the brown beatle
- Student how do the bugs dispose their waste?
- Student Tell us about it


- Bugscope Team the claws work like one of those things people use to grab things off of a shelf
- Student how sharp is their claws?
- 1:37pm
- Bugscope Team they have a part called an unguitractor on the inside
- Student what are the small hairs above the claw
- Bugscope Team you can see that the claws are pretty sharp but they are also very small
- Student what do ground beetles eat?
- Student why does it have holes
- Student what are the jobs of the claws
- Student how do they eat
Bugscope Team most are predatory , but a few are omnivores, and even fewer just eat seeds
- Bugscope Team the small hairs are likely mechanosensory


- Student what is mechanosensory
Bugscope Team it means it is for movement or feel

- Bugscope Team some eat caterpillars, snails, slugs, berries...it depends on the species
- Student do they have a mouth?

- Bugscope Team the claws help the beetle grasp its food and walk on limbs, but these beetles do not have the ability to cling to walls or the ceialing

- Student how big are they?
- Bugscope Team ceiling...

- Bugscope Team this is the underside of the beetle mouth
- Bugscope Team it has really big jaws
- Student what are the finger looking things
Bugscope Team they are the palps, they do help move aorund food, which might explain why they resemble fingers


- Bugscope Team and you can also see 2 pairs of palps
- Bugscope Team check out the scale bar in the lower left of the image, 1 um = one micron = one millionth of a meter
- Student are the little stuff in the mouth teeth
- Bugscope Team you can get some idea of the size by readfing the micron bar to the lower left


- Student are those sensory hairs

- Student what is the little rectangle in the center?
- Bugscope Team for example 304 microns is 0.304 millimeters, about 0.3 mm
- Bugscope Team yes a lot of sensory hairs
- 1:42pm

- Bugscope Team this is the center of the mouth
- Bugscope Team we are on the ant now

- Bugscope Team this is an ant comb, which does what it sounds like
- Bugscope Team and now we see the comb-like structure on the ant leg

- Student what is an ant's comb
- Student what is it used for
- Bugscope Team it uses this to clean its antennae
- Bugscope Team it cleans the ant from debris


- Student why are they so wing like?
- Bugscope Team ants use their antennae for communication much more than they use their eyes
- Student is it like feathers

- Bugscope Team they are likely wing-like so they can be tucked in and out of the way







- Bugscope Team for your antennae

- Student are the hair almost like cilia?
- Bugscope Team ants and other insects often get dirty and have to clean themselves off -- like you often see a fly doing

- Bugscope Team they are similar to cilia but I think are generally not motile

- Student is that bacteria
Bugscope Team oddly enough, we dont find too much bacteria on insects even though they are supposed to be covered in them. Bacteria are usually rodshaped and are around 2um big
- Student what do ants eat
- Bugscope Team the reason there are so many setae is because they function as part of the sensory system
- Student are those cob webs
Bugscope Team no it is some sort of dust or dirt

- Student what is the big blob?
- Bugscope Team bacteria are usually about 2 microns long and we don
- Bugscope Team 't see any here

- Student how high of a magnification does your electron microscope go to?

- 1:47pm
- Bugscope Team yeah if there were bacilli we would be able to show them to you
- Student are their any bacteria on bugs whatsoever??
- Bugscope Team we do see bacteria sometimes...
- Bugscope Team yes we occasionally see bacteria on bugs
- Student how do they multipy?
- Student How much do you usually see?
- Bugscope Team but it is usually after they die and start rotting
- Student what is a spiracle
- Student How much bacteria sorry!
- Student is that a mole
Bugscope Team this is the double stick carbon tape we use to stick the bugs to
- Bugscope Team sometimes we will see individual bacteria, and sometimes small groups, like in the grooves between the eye facets
- Bugscope Team miss smaha, your driving is adrift, do you need any help?

- Bugscope Team a spiracle is a breathing pore

- Bugscope Team here it is but the sample has drifted since we set the preset

- Bugscope Team it looks like your image is drifting, are you sure your navigation is stopped? you may need to click inside the image to stop moving?
- Teacher yes
- Student A pore like the same kiind of pore that gives humans zits?

- Bugscope Team NENAD that is one of the spiracles -- there are usually two to a segment of the body
- Bugscope Team well those are breathing pores, so not like people have
- Student what is the bill thing
Bugscope Team looks like a seta (which is setae singular)
- Bugscope Team people have skin, and insects have an exoskeleton
- Bugscope Team the exoskeleton is sort of like wearing armor would be, to us
- 1:53pm
- Student Ok its Grace, do u know what kind of little bump we were just looking at?
- Bugscope Team the exoskeleton is a shell like a shrimp shell, of chitin
- Bugscope Team miss smaha, when clicking to drive, click once to start moving, and you must click again to stop
- Teacher I can not get any thing i n focus
Bugscope Team please click in the image again, you are still moving now
- Bugscope Team Grace the bump went by so fast
- Student how does it defend its self

- Bugscope Team there ya, go it is stopped, try clicking on a preset now
- Bugscope Team Miss Smaha can you take the mag lower? That should help.




- Bugscope Team a preset is a great way to get a nice image
- Bugscope Team we were at 2500x, and it is hard to focus at that mag
- Student what insect are we looking at now? (grace)
- Bugscope Team now we see what looks like silver paint
- Bugscope Team hard to tell so far
- Bugscope Team you should be able to take the mag down lower
- Student is it silver paint? (grace)
- Bugscope Team let us know if you need help

- Bugscope Team ah cool, this is salt from wendy's!
- Bugscope Team salt!


- Bugscope Team this always looks cool
- Student Why are there holes in the salt
- Bugscope Team their salt is a little odd, it looks different from other salt strutures
- Student would this be considered grose?? (grace)
- Bugscope Team we think they put an anticlumping agent in it that makes it have those cool shapes

- Student what is the difference
- Bugscope Team Grace I don't think it is gross.
- Bugscope Team normal salt is kind of boring compared to this
- Bugscope Team normal salt will just look like cubes, wendy's salt has something else in it that gives it these cool aztec shapes
- Student do you know y the salt is always a cube?





- Student is sugar like salt? (julie)
- Student what is the stuff that gives it the shape and is it unhealthy
Bugscope Team salt, like most things, is unhealthy only is large amounts


- 1:58pm
- Bugscope Team sodium chloride forms cubes; sugar, for example, does not form crystals that are cubic
- Student why are there squaresin the squares
- Bugscope Team the way the Na and the Cl fit together gives it that cube shape
- Bugscope Team the shape comes fomr the chemistry

- Bugscope Team from
- Student is the stuff on it like dead skin? (julie)
- Bugscope Team the chemistry of the individual components and the way they fit together
- Student What is the wierdest thing you have ever seen under a microscope?


- Student why is the end of it flat?
- Student wht are they circular on the bottom
- Bugscope Team these hairs allow the insect to stick to walls and ceilings
- Student are these like suckshin cups?
- Bugscope Team we have seen some weird insects/arthropods
Bugscope Team thats what I think of them as

- Bugscope Team yes they are like suction cups

- Bugscope Team micro suction cups

- Student what do these setae help the insect with? (grace) if they are setae!
- Student why is it open on the end
- Bugscope Team they help the insect cling to ceilings

- Student why is it wrinkled up in the middle of the sucs
Bugscope Team when the insects die, they dry up and shrivel a little
- Student why are they so shriveled like a raisin

- Student what are the lines
- Student what are the wrinkles
- Student are these there arms

- Bugscope Team when they walk they can flex their tarsi and pull off of the surface that way
- Bugscope Team this is high mag, and it is hard to get perspective
- Student what is the tarsi?
- 2:03pm
- Bugscope Team the tarsi are the distal portions of the limbs -- the parts that are furgthest from the body
- Bugscope Team furthest
- Student afds
- Bugscope Team so like the forearms and 'hands'


- Student what are the circles in the backr

- Bugscope Team here you can see the cricket's eyes

- Student omfb
- Student what is behind the cricket head?
Bugscope Team that is carbon tape, used to stick to the bugs so they don't move around under the scope
- Student why are the crickets antena appear to be cut
- Student is that dust on it
- Student why are there bumps on it's eye?
Bugscope Team the eye is a compound eye, meaning it has many facets on it that each collect an image
- Student why does it look like it has a million eyes?
Bugscope Team well, that is called a compound eye, it actually does have hundreds of individual facets, each with a lens in it. flying insects often have very large compound eyes

- Teacher do they have good vision
- Bugscope Team the background is doublestick carbon tape
- Student thank you so much
- Student is there bactiera on it's face?
- Student Thank You for teaching us about bugscope!!
- Student THANK YOU
- Bugscope Team i think their vision is pretty good
- Student bye
- Bugscope Team thank you for all your interesting questions
- Student SWEET
- Student this was really great
- Student thank u so much guys\girls
- Bugscope Team you all were awesome, thanks!
- Student l8tr
- Student You guys are very nice!1
- Student thank you;)985
- Bugscope Team Oh...
- Bugscope Team Thank You!
- Student :0
- Student :)
- Bugscope Team It was fun working with you and we look forward to connecting again.
- 2:09pm
- Bugscope Team miss smaha, all the chat and images are saved on your bugscope member site: http://bugscope.beckman.uiuc.edu/members/2008-043
- Bugscope Team wow, they are gone,
- Bugscope Team that was a fast logoff
- Bugscope Team wait, miss smaha is back
- Teacher thank you so much be back next year
- Bugscope Team okay, i will start closing the session, unless you have any more questions miss smaha?
- Bugscope Team we look forward to it!
- Bugscope Team ah cool, yeah, just apply again whenever you are ready
- Bugscope Team http://bugscope.beckman.uiuc.edu/apply
- Bugscope Team ok, she's gone
- Bugscope Team let's roll!
- Bugscope Team session locked, disabled, rxl stopped