Connected on 2008-02-25 13:00:00 from Morrisville, PA, US
- 12:01pm
- Bugscope Team sample in the scope, starting vacuum
- Bugscope Team vacuum is very slow to go, may need to remove the sample...
- 12:10pm



- Bugscope Team vac ready, starting presets
- 12:16pm


- Bugscope Team yo scotteroni!
- Bugscope Team Hey Man.
- Bugscope Team making presets
- Bugscope Team What sample is this?
- Bugscope Team Oh I guess I can see.
- Bugscope Team this is a stinger from Saturday.

- Bugscope Team pupa!
- 12:22pm




- 12:28pm
- Bugscope Team trap jaw preset, and then we are done


- Bugscope Team okay, presets are done, we are ready
- Bugscope Team hello high point, welcome to bugscope!
- Bugscope Team Hello High Point!
- Bugscope Team where are you from?
- Guest Hello. We are looking forward to this.
- Bugscope Team oh hey, you are from high point school
- Bugscope Team you've got a session on the 28th, awesome.
- Guest Sparta, Wisconsin
- Bugscope Team i just gave you control of the scope. we are waiting for the school to login, but we've got about 15-30 minuites, so you are welcome to practice now
- Bugscope Team Looks like Alex just gave you control if you want to test for a few minutes.
- Guest thanks
- Bugscope Team you should see controls on your right (magnify, navigation, focus and adjust)


- Bugscope Team then there are preset on the bottom right. you can click on a preset to force the scope to goto an intersting location we picked for you


- Bugscope Team otherwise, you can just drive the scope around anywhere you want to

- Guest that is so easy and cool


- Bugscope Team hi suziq56, welcome to bugscope!
- 12:33pm
- Bugscope Team where are you from suziq56?




- Guest I am from Raleigh, NC.





- Bugscope Team cool, are you here for practice?
- Bugscope Team There is a college in NC called High Point as well.
- Guest I feel comfortable already if they want to take over
- Bugscope Team that is great high point, glad you are digging it, any questions?
- Guest I am just observing out of curiosity.
- Bugscope Team ok, no problem suzi156
- Bugscope Team Cool! You are welcome, of course.


- Bugscope Team That was a leaf mimic ant.
- Bugscope Team the one that looked like the flying nun.
- Bugscope Team high point, if you notice any lag during your session, then you should stay away from "click to drive" and stay on "click to cneter". however, your compatibility test was excellent, and your network speed was awesome, so i don't forsee any problems

- Bugscope Team now you can see one of the bee eyes, with lots of setae sticking out of it
- Bugscope Team "click to drive" sends live video to your browser, whereas "click to center" sends incremental images, which are much less data
- Guest The controls are working just fine
- Bugscope Team awesome


- Bugscope Team Hi Jon!
- Guest What do the setae do, protect the eye?
Bugscope Team it is thought that the setae on the compound eye are mechanosensory, so they help the insect to sense air speed and air direction while flying. that's how they move so fast when your hand is about to hit them!

- 12:38pm
- Bugscope Team hi all

- Bugscope Team they move in response to the wind and the bee can feel that
- Bugscope Team just like hairs on your arm feel the wind

- Bugscope Team insects/arthropods have lots of setae that help them keep in touch with the outer world
- Bugscope Team since they have an exoskeleton


- Guest fire drill have to leave now thanks
- Bugscope Team oops wow
- Bugscope Team ok, thanks for loging in high point! see you on the day of your session

- Bugscope Team suziq56, i just gave you control of the scope
- Guest OK I will go for it.
- Bugscope Team you should see controls now, on the top right (magnify, navigation, focus, adjust) and then presets on your bottom right
- Bugscope Team You can move, you can select a preset from one of those on the right, you can change mag...

- Bugscope Team and you can also refine focus and change the contrast/brightness


- Guest I can't seem to move it where I want to.
- Bugscope Team hmm, what happens when you try click to center?
- Bugscope Team does it say anything like: can't execute command?
- Bugscope Team ah, i see you are moving with "click to drive"
- Guest I got it now.
- 12:43pm
- Bugscope Team click to drive means you have to click once to start moving. then click aagain to stop
- Bugscope Team click to drive is also hard to control if your network is slow

- Bugscope Team if you are seeing lots of lag, then try click to center instead
- Bugscope Team yeah that is the trick -- click to stop
- Bugscope Team oops now we are off the stange.

- Bugscope Team make sure when you use click to drive that you click again to stop
- Bugscope Team if the image isn't updating fast enough, that means your internet is a little slow
- Bugscope Team if that's the case, then use click to center instead. click to center is very good too.
- Bugscope Team sometimes the stage will reach its limit and we have to reset it
- Bugscope Team much easier to get fine control with click to center

- Bugscope Team Yay Annie!
- Bugscope Team if you want to go to an interesting location, click on one of the presets. we set those up for ya

- Guest Do you do any sessions with butterflies or catetpillars?

- Bugscope Team yep!

- Bugscope Team but they will usually be parts of them since they are so big
- Bugscope Team like a portion of wing, and the body
- 12:48pm
- Guest This is so interesting but I gotta get back to work. I am going to read up more about this so I can do this with some students here.
- Bugscope Team thanks for joining suzi56!
- Bugscope Team sounds great SuziQ we will be glad to have you here
- Bugscope Team awesome, just fill out an apllication when you want to do it.
- Guest back again. the 28th we will be doing this with montessori students grades 1-4. they will love this.
- Bugscope Team cool. looking forward to it!
- Guest any suggestions for specimens to look at that day?
- Bugscope Team are you going to let the students login and chat?
- Bugscope Team well, we can provide you with a sample of bugs if you didn't send us one. we are good at picking out bugs.
- Guest we will have them asking the questions but the teacher will probably type since we are hooking it up to the infocus projector
- Guest we planned on using your bugs
- Bugscope Team ok, no problem, we will make sure to get you a good sample. we always try to pick good bugs. scott and cate usually prep the sample, and they are experts at it
- Bugscope Team Cate is good.
- Bugscope Team okay, teacher doing the typing is fine. it is your decision as the teacher to decide how to run your session.
- Bugscope Team i will say though, that it is often very interesting for the students when they can talk to us directly.
- 12:54pm
- Bugscope Team Sometimes with younger students an aide types the questions and a teacher drives, or vice versa.
- Guest we will try to have kids write to you
- Bugscope Team oh, depends on age too. younger kids might not type so well
- Guest their spelling may be questionable
- Bugscope Team but we love talking directly with the kids
- Bugscope Team ours is too
- Bugscope Team i'm a terrible speller!
- Bugscope Team just ask scott
- Guest i am on the phone with the teacher right now. she and the kids are excited
- Bugscope Team cool, is that doug geiwitz?
- Bugscope Team a part of bugscope that seems very effective is having the kids write and get responses from us; they like to be acknowledged
- Guest yes
- Guest how many of you are current students?
- Bugscope Team I am a PhD student in entomology
- Bugscope Team annie is a PHD student, in entomology, the rest of us are staff at the U of I
- Bugscope Team just Annie. I guess we are all perennial students
- Guest what a great way to interact with young kids.
- Bugscope Team okay, it's about time for the school to login. no word from them yet. scott i'll call them in 5 minutes
- 12:59pm
- Bugscope Team it is the most fun we have in a week
- Guest how do you use this technology in your work
- Bugscope Team we train grad students and postdocs, mostly, to use the microscopes to do their own research
- Bugscope Team this is one of the microscopes
- Bugscope Team we are a support group for the Beckman Institute, so we setup, train and fix all the EQ that is used by studetnts, and faculty: microscopes, scanners, etc.
- Guest what is the max magnification
- Bugscope Team some look at bugs others at bones
- Bugscope Team we have a lot of engineers looking at all kinds of stuff
- Bugscope Team the remote access technology is used less frequently, but sometimes it is involved in lectures
- Bugscope Team we can go over 800,000x, but meaningful images no more than about 200,000x
- Guest thanks for the introduction and have a great session today. see you on the 28th.
- Bugscope Team we are looking forward to it, thanks!
- Bugscope Team Thank You!
- Bugscope Team thanks bye
- 1:04pm
- Bugscope Team hi carolyn, welcome to bugscope!
- Bugscope Team Welcome to bugscope Mrs Schmitt!
- Bugscope Team hi there, from PA! you are looking at the stinger on a fire ant
- Bugscope Team mrs. schmitt, you now have control of the scope. but at any time, we can give control to any student. just let us know.
- Bugscope Team if you have any questions about what you are looking at, or how to control the scope, just ask away.
- Bugscope Team we are here to help
- Bugscope Team that was me with the brightness
- Student Tom: How long have you been scientist?
Bugscope Team I have been in graduate school for five years...I don't know when I became a scientist though.
- Bugscope Team scott, jon and annie are scientists, i'm a computer geek
- Bugscope Team or if I am one now!
- 1:09pm
- Bugscope Team Yeah Annie is a scientist.
- Bugscope Team she will have to admit it sometime
- Student Ben; What is the electron microscope used for?
Bugscope Team we use the scope to see fine details that we can not see with normal light microscopes
- Bugscope Team eventually


- Bugscope Team this electron microscope is used to image the surfaces of 3-dimensional, small, samples
- Bugscope Team the micrope can magnify up to 800,000x, but normally we image things much less than that. for bugs, we use 25x-20,000x magnification
- Teacher joe : what bug is this
- Bugscope Team we have another electron microscope (a transmission electron microscope) that allows us to see ultrathin samples
- Student Teacher: What is the unit of measurement used in the lower left hand corner of the screen
Bugscope Team good quesiton. the unit of measurment is a micron, one millioth of a meter
- Bugscope Team this is a fire ant abdomen
- Bugscope Team this is a fire ant, the abdomen, to the north is the stinger
- Bugscope Team micrometer or micron
- Bugscope Team one thosusandth of a millimeter
- Teacher ?
- Bugscope Team thousandth


- Bugscope Team these were all donated by the entomology department at the university of illinois
- Teacher Nick: What other types of things beside bugs do you magnify?
Bugscope Team virtually anything we can fit in the scope we can magnify
Bugscope Team bacteria, microchips, paint chips all kinds of stuff
- Bugscope Team be sure to try some of the presets
- Student Gianfranco: What is the most interesting bug that you ever magnified?
Bugscope Team there are always interesting things to look at on bugs, but i like it when there are ticks on bugs. bugs on bugs!
- 1:14pm
- Teacher MIke are you using the bugs we sent you?
Bugscope Team no, i'm sorry but we couldn't prepare your bugs today, so we are using a sample that we used this past weekend, for an insect fear festival
- Bugscope Team people look at cells, carbon nanotubes, sand, dirt, rocks, meteorites, bacteria, salts, samples they have made out of silicon, PDMS...
- Bugscope Team PDMS is a rubbery plastic
- Student Teacher: How do we access the presets?
Bugscope Team if you are logged in as the teacher, just click on any preset
- Bugscope Team click on one, on the right
- Bugscope Team the teacher will need to do it
- Bugscope Team mrs. schmitt, if you are on the computer as "student" let me know, and i can give control over to you
- Bugscope Team the teacher has a star next to get name indicating that she has control of the microscope

- Bugscope Team next to her name I should have said
- Student Teacher: Are there any major discoveries in medicine that have been made utilizing the electron microscope?
Bugscope Team I think that people discovered introns using scanning electron microscopy...that has important implications for all areas of biology, including medicine.

- Bugscope Team some diseases can be seen using the electron microscope that cannot be seen other ways
- Teacher alex:why is are alien ant look so fuzzy
Bugscope Team the image is out of focus, you can adjust focus with the controls, try it and let m eknow if you need help
- Bugscope Team virus structures can be determined, for example
- Bugscope Team transmission electron microscopy has been around since the 50s; it was invented in the 30s.
- Bugscope Team Introns are sections of junk DNA that the cell cuts out before translating the DNA code to make proteins
- Student Megan: Are you scared of any kind of bugs?
Bugscope Team I hate horse flies...ugh, I HATE them
- Teacher pat: what kind o bug intrest u the most
Bugscope Team I study longhorned beetles...wood-boring beetles with long antennae
- Bugscope Team scanning electron microscopy -- what we are doing now -- was not commercial until 1965
- 1:20pm
- Bugscope Team I don't like chiggers
- Bugscope Team mrs. schmitt, when focusing, try going one direction, if it gets worse, go the other way.
- Bugscope Team ah, yeah, there ya go!
- Bugscope Team nice!

- Bugscope Team this is a leaf mimic ant
- Teacher Taylor: What is the most amount of legs on a bug have you seen. What bug?
Bugscope Team That's a trick question! All insects have 6 legs!
- Student Allison: Once you put a bug under the microscope, how long does it take to start seeing the image?
Bugscope Team not long maybe five minutes. In most cases it takes a long time to get an object like a bug ready for the scope

- Bugscope Team this is another ant from Argentina
Bugscope Team well, milipedes have hundreds of legs on them, that's a lot, huh?

- Bugscope Team we have seen close to 100 legs on some millipedes

- Bugscope Team if we consider other arthropods to be bugs
- Student What is a chigger
Bugscope Team red bugs that hang out in spanish moss in the south
- Bugscope Team true bugs are insects and have only six legs as adults
- Bugscope Team But, we shouldn't because bugs are a type of insects. All bugs are insects, but not all insects are bugs!
- Student Allison: What do you have to do to prepare the bug for the scope?

- Bugscope Team some insects can have more than six legs before they become adults
- Bugscope Team AND, not all arthropods are insects
- Student Are chiggers poisonous?
Bugscope Team They are not poisonous per se. They secrete an enzyme that dissolves your cells and that most people have an allergic reaction to
- Bugscope Team A chigger is a mite! It is not an insect at all!
- Bugscope Team chiggers are super tiny
- 1:25pm
- Teacher how do you move the miroscope down into his mouth
- Bugscope Team and we have never had any in the 'scope that I know of
- Bugscope Team those red bugs are often spider mites
- Bugscope Team you can use the click to center feature
- Bugscope Team since you are so close



- Teacher Molly: how did you decide to become a scientist.
Bugscope Team I always liked being outdoors and learning about the natural world around me. When I was in high school, I decided to make a career out of being outside and looking at insects
- Bugscope Team I was in danger of becoming an English teacher and decided to add biology to my major in college
- Teacher Carly: How high can we magnify this bug to 300,000
- Bugscope Team 'cause I thought English teachers were kind of weird.
- Student Abigail: What are the holes on the stinkbug called and what are they used for?
Bugscope Team Which holes? There are lots of hole on a stinkbug, some that the bug uses for breathing, some that it uses for eating, etc. etc.
- Bugscope Team the scope can magnify up to 800,000x, but most insects don't show up well much past 20-30 thousand times.
- Bugscope Team there is not much to see on this bug at that high magnification
- Bugscope Team some of the holes are spiracles


- 1:30pm
- Bugscope Team spiracles are used for breathing, and sometimes they do also perform other functions such as releasing toxins or smells
- Bugscope Team insects have lots of defenses that are carried out in a variety of ways

- Student Nidhi: How many different microscopes are there?
Bugscope Team there are light microscopes, electron microscopes, and scaning probe microscopes each with of those three have a bunch of different sub catagories depending how they used. This is a scanning electrom microscope which is a version of an electrom microscope
- Bugscope Team many insect defenses are chemicals released to keep ants away
- Teacher Kayle: Is this really an eye?



- Bugscope Team there are many different kinds of microscopes


- Bugscope Team yes this is a compound eye

- Bugscope Team with tiny facets on it called ommatidia






- Bugscope Team this is an eye on a bee pupa though...it is not fully developed
- Bugscope Team a compound eye has hundreds of eye facets, or ommatidia, each facet has a lens and can get an image and send it to the bug brain

- Bugscope Team this bee pupa is a little wrinkly even though we critical point dried it



- Student ]
- Bugscope Team sometimes we can see an eye that has dried and broken, and we can see that the ommatidia really are little lenses


- Student Can we get the stingless bee?
- Bugscope Team if you look closely at the eye of the bee pupa you can see the ommatidia

- 1:35pm

- Bugscope Team please
- Teacher we are trying to click on preset 4 but we keep getting a warning

- Student Kate: Does this microscope enable you to see an actual cell within a body of a bug?
Bugscope Team this scope is designed to see the surface of objects, so it is hard to sea individual cells, but sometimes we see bacteria on them which are single cell organisms


- Student Alek: How much does this type of microscope cost?
- Bugscope Team hmm, try again, i think the scope is fine
- Bugscope Team I cannot get the 'scope to move either.
- Bugscope Team i don't see any error on the scope
- Bugscope Team about $600,000








- Bugscope Team it has its own room, its own water and air and nitrogen and electrical power, and AC




- Bugscope Team okay, i think i fixed the problem. try again mrs schmitt

- Bugscope Team yay!


- Bugscope Team stingless bee
- Teacher yeah
- Teacher Julia: why is it so scrunched up?
Bugscope Team when we prepair the bug for the scope the muscles inside shrink making the legs and sometime the body curl.
Bugscope Team sometimes the bugs get very dried out when we put them in the scope, this may cause the scrunching you are seeing





- Student Ethan: What is the biggest bug you have ever seen under the microscope?
Bugscope Team I have looked at parts of beetles that are about 2 inches long
- 1:40pm
- Bugscope Team Insects often fold up when they die



- Bugscope Team we have seen parts of walking sticks in the 'scope
- Student Can you use the electron microscope to view live specimens?
Bugscope Team well, sometimes the insects will survive being in the scope. scott has open the scope only to find a bug walk out of there!

- Bugscope Team they are pretty big


- Bugscope Team we have used it to image live samples but not on purpose



- Bugscope Team there is a vacuum in the specimen chamber
- Bugscope Team The beetles are two inches long, the beetle parts are smaller
- Student Connor: How old is this particular microscope?
- Bugscope Team this is as old as bugscope, a little over 9 years
- Bugscope Team prepare
- Bugscope Team it's that BSE starting to kick in
- Teacher mason do you have more than one eletrone scope?
- Bugscope Team yes, we also have a transmission electron microscope
- Teacher Nick: how are you doing?
- Bugscope Team we have a scanning electron microscope and a TEM, as Alex says
- Student Ryan: About how fast are the electrons traveling? And what year was the electron microscope discovered?
Bugscope Team the first electron microscope prototype was built in 1931. as far as the speed of electrons, that is a difficult subject. of course it's about the speed of light. but there is a branch of science that says you can't measure the exact speed or location of an electron
- Bugscope Team 1931 for TEM
- Teacher we need be just about done now THANK YOU
- Bugscope Team I think they travel as fast as light
- Bugscope Team Thank You!
- Student Thank you so much for your time!!!!!!
- Bugscope Team the electrons are functioning like light here
- 1:45pm
- Bugscope Team 1938 for the SEM
- Bugscope Team Heisenberg. Actually you can measure the speed but not the location or the location but not the speed
- Bugscope Team ah, yes, you can't measure both at the same time. but individually you can. thanks scott
- Teacher you were great!! we enjoyed the session
- Bugscope Team See you next time! THANK YOU!
- Bugscope Team thank you mrs schmitt!
- Teacher I will submit again next year, did you receive my bugs???
- Bugscope Team we did get your bugs, but we had some problems getting them together, so we used our own sample today.
- Bugscope Team we will make sure to prep your sample next time you send us bugs
- Bugscope Team Oh we did receive your bugs, and on time. But Cate is out sick today, as am I, so we did not get to prepare them. I am sorry.
- Teacher We will try again next year, can I use the same propsal??
- 1:51pm
- Bugscope Team well, sure, but you will need to fill out another application. it's easy though, just goto http://bugscope.itg.uiuc.edu/apply
- Bugscope Team Just copy the proposal and resubmit using a new application so we can keep track for our records.
- Bugscope Team we catalog the session by year
- Bugscope Team you are something like our 336th session ever
- Bugscope Team not counting test sessions
- Teacher thanks again!!!
- Bugscope Team Yay!
- Bugscope Team ok, i'll close the session, and put the sample back in the fouke decaliter
- Bugscope Team ok, nice session everyone
- Bugscope Team rxl stopped, session disabled
- Bugscope Team bye bye!