Connected on 2007-12-20 16:30:00 from , AZ, US
- 3:52pm
- Bugscope Team setting up
- Bugscope Team very good samples today


- 3:59pm


- 4:06pm



- 4:12pm


- Bugscope Team This is the best set of samples we have ever had.
- 4:17pm

- Bugscope Team Okay we are ready to roll.





- 4:24pm
- Bugscope Team Leaving the rasper exposed.
- Bugscope Team this is the recurved spine side and the upper side is more file-like.
- Bugscope Team Welcome Aaron!
- Bugscope Team Hi Mr Miller!
- Bugscope Team I was telling Chas that this is the best set of samples we have ever had in almost 9 years.
- Bugscope Team Cate sonicated them this morning before mounting them.
- Bugscope Team SoniCate.
- Bugscope Team Yay Claudi!

- Bugscope Team Hello
- Teacher We are in.
- Bugscope Team This is the part of the mouth that sticks into your skin.
- Bugscope Team awesome
- Teacher Thank you about the props for our samples. We tried to get the best we could

- Bugscope Team Claudi is using some strange German browser we don't recognize.
- Teacher So, this is the tick?
Bugscope Team This is the underside of the head, showing the mouthpart that would stick into your skin with all the recurved spines that keep it from coming out
- Bugscope Team You're welcome to let students login simultaneously from more computers if they're available
- Bugscope Team yes it is
- Bugscope Team Sorry
- Bugscope Team please let us know if you have any questions and feel free to drive around
- Teacher What are the circular shapes on either side?
Bugscope Team Those look to be pedipalps, auxiliary mouthparts
- Bugscope Team I think all of the samples are on and have presets associated with them.
- Bugscope Team What class is this? My mom is watching and is fascinated.
- Bugscope Team they are some sort of sensory palps, probably
- 4:30pm
- Bugscope Team the eyespots will be on the dorsal side of the head, and we will not be able to see them
- Bugscope Team those arched pieces of the head will separate when the central portion sticks into your skin.

- Bugscope Team this is why they don't come out so easily

- Bugscope Team Kind of like the barbs on a harpoon
- Bugscope Team Claudi say Hi to your mom for us.
- Bugscope Team yeah like a harpoon
- Bugscope Team it's 11:30 there. p.m.
- Bugscope Team Scott, I had to use F11 and I cannot see the schools application. Mom says Hi back.
- Bugscope Team if you get lost take the mag down


- Bugscope Team this is the doublestick carbon tape
- Teacher So, we click drive to move the scope? We seem to be spinning out of control
- Bugscope Team Click again to stop the driving.
- Bugscope Team it is a little tricky -- you click to start driving and then click to stop
- Bugscope Team so sometimes you don't realize you have clicked either way.

- 4:35pm
- Bugscope Team Click again inside the image somewhere that is. You click once to start -- once to stop. Once you stop you can return to somewhere interesting using one of the presets at the lower-right corner of the page



- Teacher we are checking out the presets first

- Bugscope Team Whoops, I think we're still driving...



- Bugscope Team I just stopped it. Go ahead and try the preset again
- Teacher yes, oops

- Bugscope Team true bug proboscis
- Bugscope Team this is where the sheath has separated and we can see the lancet -- the sharp part -- that is inside
- Teacher thanks for the movement advice. we were a little out of control
- Bugscope Team if you take the mag down here you can see where you are
- Teacher what is behind the lancet. It looks like spider web



- Bugscope Team if you were to drive north you would see the eyes and the origin of the mouthpart
- Bugscope Team that is mold
- Teacher ooh, cool
- Bugscope Team the stuff that looks like web is mold
- 4:40pm
- Bugscope Team yay Smiley is back!
- Teacher what are the four holes on either side of the lancet
Bugscope Team I believe those are empty holes where the legs should be. They must've gotten knocked off during preparation or during travel
- Bugscope Team mosquitos have a similar apparatus



- Bugscope Team those holes may be where the limbs are missing
- Bugscope Team you are getting more proficient at driving
- Bugscope Team Annie!
- Bugscope Team hooray, it worked
- Bugscope Team I told them this was a crab...
- Bugscope Team I am just back from California
- Bugscope Team You didn't stay long, apparently
- Bugscope Team I am explaining my mom and translating as we go...yeah!!!
- Bugscope Team tell your mom I am trying to get Annie to say NO NO this is a true bug



- Bugscope Team right right---this is a hemiptera of some description
- Bugscope Team Yay SoniCate.



- Bugscope Team Annie are you in town here?
- Bugscope Team Nope, I am in Kentucky

- Bugscope Team the eye!

- Bugscope Team OOF
- Bugscope Team oh yeah good job focusing
- 4:46pm
- Teacher why thank you
- Teacher What is that stuff on the eye?
- Bugscope Team juj
- Bugscope Team juju
- Teacher ???
- Bugscope Team juju is our catch-all term for dirty and junk that we see on the insects
- Bugscope Team it's some slime that might have come out of the bug when it died
- Teacher ok that's what we thought. we'll use it too

- Bugscope Team this is pretty rad too
- Bugscope Team my mom is thrilled...I showed her the omatitia on the compound eye
- Bugscope Team double clipping jaws
- Bugscope Team cool--what is this?!
- Teacher so there are two sets of jaws?
- Bugscope Team your mom is staying up late for this
- Bugscope Team scorpion
- Bugscope Team it's a baby lobster
- Bugscope Team awesome!
- Teacher so the hairs are called setae?
- Bugscope Team thats right
- Bugscope Team yes
- Bugscope Team some of them are mechanosensory, as these likely are
- Bugscope Team Interestingly daddy-long-legs spiders have a very similar pair of pincers due to their being in the crustacean family rather than being true spiders
- Bugscope Team and some are chemosensory
- 4:51pm
- Bugscope Team Actually, daddy longlegs are arachnids, but not spiders
- Teacher is that connective tissue in the mouth?
- Bugscope Team some chemosensory setae have receptors for only one chemical, and some can sense more than one chem.
- Bugscope Team not true spiders anyway


- Bugscope Team Whoops, sorry for the mis-information.

- Bugscope Team ;)

- Bugscope Team it is confusing


- Bugscope Team that is probably my fault since I am convinced that DDLs are crabs

- Bugscope Team opiliones
- Bugscope Team It's also confusing that the daddy long-legs name applies to two both Opiliones and Crane flies
- Bugscope Team my mom thinks it is awesome. enjoy the rest of this session. see you guys next year
- Bugscope Team harvestmen too
- Bugscope Team Thanks Claudi!
- Bugscope Team that is confusing---and that is one of the problems with common names

- Bugscope Team I believe harvestmen and opiliones are one and the same.

- Bugscope Team Enjoy Germany, Claudi!!
- Bugscope Team Yes, as far as I know, harvestmen and opiliones and daddylonglegs are all the same

- Bugscope Team Claudi is r-u-n-n-o-f-t.
- Bugscope Team I know what this is!!!!



- Bugscope Team With the pore showing
- 4:56pm


- Bugscope Team there is a longhorned beetle with a stinging antenna tip with a pore like this

- Bugscope Team yeah Cate made the sample and did a great job, with drawings
- Bugscope Team OOF
- Bugscope Team and they sting too!
- Bugscope Team Oh I want to see that
- Bugscope Team Nice, Mr M. You are a pro.
- Bugscope Team those drawings were awesome i know
- Bugscope Team the eightball esp.
- Teacher what is OOF?
- Bugscope Team out of focus
- Bugscope Team well it's not now
- Bugscope Team Out Of Focus
- Bugscope Team they look exactly like this! It is really remarkable
- Teacher excellent job preparing these. They look awesome?
- Bugscope Team Yeah, they are really nice!
- Bugscope Team yes they do
- Bugscope Team could be crystallized poison
- Bugscope Team Looks like something in the canal here dried up
- Bugscope Team spider fangs are similar when you get to see them
- Teacher what are the small black dots here?
Bugscope Team not really sure what these small pores actually do
- Bugscope Team tiny pores




- 5:01pm
- Bugscope Team Mr M it is hard to focus here because we are pretty far from the polepiece and I left a tiy bit of astigmatism in the image, apparently

- Bugscope Team xtals
- Teacher no worries
- Teacher do you know what kind of crystals?
- Bugscope Team this is on the fly eye; to the right is some of the vestiture
- Bugscope Team Looks more elongated than regular salt, so it might be crystals of something else
- Bugscope Team we see them sometimes but yeah like Chas says they are likely not NaCl.
- Bugscope Team this fly did not fare too well
- Bugscope Team but this part looks great
- Bugscope Team if we were to do EDS we might be able to figure out what elements are in the extals
- Bugscope Team we will save this sample and might be able to find out tomorrow
- Bugscope Team EDS is energy-dispersive spectroscopy -- elemental analysis
- Bugscope Team EDS stand for energy dispersive spectroscopy. The bombardment of the sample by electrons, how we're imaging, also produces X-rays. The exact wavelength of the X-rays produced can tell us about the atomic composition of what we steer the beam at
- Teacher umm, what is vestiture, looks like french fries
- Bugscope Team hehe french fried
- Bugscope Team we can detect either the wavelength or the energy of the x-rays
Bugscope Team These are actually equivalent, but you can sense them in two different ways. One physically spreads the wavelengths out like a prism separates the colors of light. Another counts the number of electrons liberated by the X-ray hitting the detector, sensing the energy deposited
- Bugscope Team fries
- Bugscope Team it's like fur on the head between the eyes
- Teacher cool
- Bugscope Team vestiture like a dress is a vestido; it's like the dressing on the head
- 5:06pm






- Bugscope Team now you can see where the larger setae, like bristles, were
- Bugscope Team Now you can see some moth scales lying on the compound eye there.
- Teacher what are the craters on the vestiture?
Bugscope Team Those look like empty pores that large hairs, "setae", would have been coming out of
- Teacher ooh, moth scales
- Bugscope Team those are pores where the setae were
- Bugscope Team sockets, I guess
- Teacher which way is the mouth?
Bugscope Team To the lower right
- Bugscope Team They have a very distinctive shape and texture, making them easy to identify
- Bugscope Team it would have been to the south but it is way gone
- Bugscope Team the antennae are missing, as are the mouthparts

- Bugscope Team there's a mouth for you
- Bugscope Team if you take the mag down you can see where you are
- Teacher why are there setae in the mouth?
- 5:12pm
- Bugscope Team for tasting/smelling

- Bugscope Team some of them could be chemosensory, like taste buds
- Bugscope Team different setae have different functions as you have gathered
- Bugscope Team and some could just filter the food

- Bugscope Team keep it from crawling back out of the mouth
- Bugscope Team some, like on an eye, will tell the insect which way the wind currents are going
- Bugscope Team others are sensitive to vibration, like on a spider

- Bugscope Team ladybugs eat other bugs
- Bugscope Team here you can see the jaws, like crossed hinged gates
- Bugscope Team above

- Bugscope Team the big setae are prob'ly the mechanosensors
- Bugscope Team mandibular palps
- Bugscope Team the stalk like things
- Teacher are those small legs around the mouth?
Bugscope Team Those are called pedi-palps. They usually act in a complex coordinated way to help manipulate the food and shove it into their mouths. They do a lot of the functions we would use our tongue for.
- Teacher you read our minds
- Teacher do the palps break up food our just feel?
Bugscope Team palps taste and feel, but don't "chew"
- Bugscope Team the centers of the ends of the palps, as you can see on the left, have what we think are chemosensory setae in them

- 5:17pm


- Bugscope Team ladybugs also have larger maxillary palps that look like vacuum cleaner nozzles
- Bugscope Team here we go


- Bugscope Team now you can see the chemosensory setae inside
- Teacher it looks like a cactus fruit or bee hive

- Bugscope Team this is kind of cool too
- Bugscope Team yeah they have vacuum cleaner palps and bee hive palps
- Teacher where are these setae located
- Bugscope Team this is cool
- Bugscope Team this is near the head i think
- Bugscope Team spiders almost always have these totally awesome-lookin' setae
- Teacher what do these setae do?


- Bugscope Team I think they are vibration sensing


- Bugscope Team i don't know much about spider hairs
- 5:22pm
- Bugscope Team but Chas had brought up the idea of the shape having the ability to shed web silk


- Bugscope Team they don't have flat surfaces for something sticky to adhere to.

- Teacher Are those little bugs on the spider? look like caterpillars or leeches
- Bugscope Team I think those setae were on a leg, but spiders have similar setae everywhere


- Bugscope Team the round things are four of the eyes


- Bugscope Team I think we are looking at fungal hyphae on this tick claw
- Teacher wow, fungus
- Teacher how many claws does it have total
- Teacher is a tick an arachnid?
Bugscope Team yes, it is. It was eight legs. Mites and chiggers are arachnids too. And scorpions and horseshoe crabs.
- Bugscope Team I think it would have 16
- 5:27pm
- Bugscope Team if it has two claws on each arm
- Bugscope Team but it does have a set of extra claw like things that it uses to perch
- Bugscope Team like to perch on leaves

- Bugscope Team that should have read 'has', not was

- Bugscope Team resolution standard built into the wings of a cicada
- Bugscope Team the astigmatism is not that bad
- Bugscope Team you can see from the micron bar that the dots are submicron -- they are nanoscale
- Teacher is that a setae or a wing vein?
- Bugscope Team it's a seta on a wing vein
- Bugscope Team if you take the mag down a bit you can see


- Bugscope Team there...

- 5:32pm
- Bugscope Team that is pretty cool
- Bugscope Team I didn't expect to see that detail on a cicada
- Teacher what is that ball on the setae
- Bugscope Team juju
- Teacher that dang juju
- Bugscope Team yeah it is some oily substance we don't know where it comes from


- Bugscope Team this is a pattern I don't remember seeing in the past

- Bugscope Team very beautiful architecture to the scale on the butterfly wing

- Bugscope Team have a great night everyone! Gotta go for now.
- Bugscope Team happy bugscoping!!!!!

- Bugscope Team really? what color were the wings? grey?
- Bugscope Team this is another moth's eye
- Teacher it looks like fingerprints on the eye
- Bugscope Team well I guess this is the first moth we have see today
- Teacher brown with blue and orange
- 5:37pm
- Bugscope Team moths seem to have the finest substructure on their eyes
- Teacher i wonder why? seeing at night?
- Bugscope Team I have this idea that the microarchitecture of the scale is responsible for the color




- Bugscope Team Mr M maybe so. I had been thinking UV but maybe they are like rods/cones, whichever one is best at night
- Bugscope Team rods?
- Bugscope Team rods i believe
- Bugscope Team bubba
- Bugscope Team Oops we lost Mr M.
- Teacher wow this is cool





- Bugscope Team 200,000x
- Bugscope Team now the working distance makes a big difference in the res.
- 5:42pm
- Teacher we were just trying to zoom in on the rods
- Bugscope Team if we were closer to the electron source we would get better imaging
- Bugscope Team sorry I placed us about an inch away
- Bugscope Team for shame scott
- Teacher Well thanks for your time. We need to sign out. Thank you for staying late
- Bugscope Team you can see that the rods are around 100 nm across -- smaller than the wavelength of UV, even.
- Bugscope Team we hope you had fun
- Teacher Merry Christmas and happy new year from the kids. This was the best ever, after met
- Bugscope Team scott is trying to make the image better
- Teacher cool looks awesome
- Bugscope Team I can bring it closer...
- Teacher We saw those closer images thanks for doing that!
- 5:48pm
- Bugscope Team when we get too close the electron beam affects the sample
- Bugscope Team but that is a little better

- Bugscope Team now I probably messed up all of the rest of the presets
- Bugscope Team yep
- Bugscope Team but thats ok
- Teacher Well that's okay our session is about over.
- Teacher I'll be applying again. The kids really liked it!
- Bugscope Team Well perfect, then. Mr M we had a great session, Thank You.
- Bugscope Team Yeah be sure to come back.
- Bugscope Team Merry Christmas to you as well.
- Bugscope Team You know the images and text are available on the page under your login.
- Bugscope Team And email us if you have any problems getting to them.
- Bugscope Team I am shutting down now...
- Teacher Us too.
- Bugscope Team cool!
- Bugscope Team Thank You!