Connected on 2008-05-09 08:30:00 from , NY, US
- 7:25am
- Bugscope Team hello katy!
- Teacher hello! this is Katy, the teacher, I'm just setting up their computers and then, if you don't mind, I'll look around to get an idea of what we
- Teacher what we'll look at.
- Teacher they cannot come in (homeschool group) until 9:30am...
- 7:30am
- Bugscope Team Katy we are making the presets now; it'll take a few minutes...
- Teacher sounds good. we're supposed to have 7 students...and me! take your time...
- 7:37am
- 7:43am
- 7:50am
- 7:56am
- Teacher this is so cool...thanks for all of the extra specimens. I miss SEM!!!
- 8:01am
- Bugscope Team you should have your pick from a lot of presets/insects
- 8:10am
- Bugscope Team Katy you can try driving now if you want.
- Teacher thanks-awesome...I'll play around now...
- Bugscope Team Let us know if you have any trouble.
- Bugscope Team the Adjust buttons are for contrast/brightness.
- Bugscope Team Focus is stepwise, in one direction or the other.
- Bugscope Team When you click to drive you also need to click to stop.
- Bugscope Team nice...
- 8:15am
- Teacher most of the SEM I did back in the day (not too long ago) was not digital and we used polaroid. so, I'm impressed!
- Teacher this is an environmental SEM, right? but are we using it as such?
Bugscope Team we are using it as a regular SEM and not as an ESEM right now
- Bugscope Team like type 55 film and sodium sulfite?
- Teacher yes, and the green scanning screen in a dark room. ;) but when I moved on to grad school, things were digital.
- Teacher (all liver or liver cells, or our 3-d liver model)
- Bugscope Team you cultured liver cells?
- 8:20am
- Bugscope Team hepatocytes
- Teacher yes, but also looked at sections after partial hepatectomy during regeneration and also after seeding cells into our bioreactor to look at cell organization.
- Teacher I cultured hepatocytes, but my focus was on the endothelial and stellate cells and their role in angiogenesis and fibrosis, etc.
- Teacher now I'm teaching! ;)
- Teacher about research...
- Bugscope Team it is interesting that parts of the liver die, like from drinking alcohol, and scar tissue forms, and then the liver regenerates but has to grow through the scar tissue
- Bugscope Team is what you are doing today normal or a sideline?
- Teacher yes! but eventually cannot. the stellate cell population "activates" or changes phenotype and becomes myofibroblast-like...this in turn causes the scarring...and prevents O2 from getting to the cells, etc.
- Teacher during normal regeneration (after parts of the liver are cut out), the stellate cells seem to partially and reversibly activate when needed but they revert back to their quiescent state. They are the "communicators" and architects of the liver.
- Teacher these bugs are much cooler though. ;)
- Bugscope Team thats why we look at bugs and not something else, because they are much cooler to kids
- 8:26am
- Bugscope Team but sometimes, like today, we put other things in the scope like salt
- Bugscope Team Hello all!
- Teacher is that "wendys" as in the restaurant or as in a person you know? ;)
Bugscope Team the restaurant. they have different salt then we have seen before, ther eis more structure to it
- Teacher Hi Annie!
- Teacher the students should be arriving as we speak.
- Bugscope Team Hi Annie!
- Bugscope Team Annie is our entomologist, connecting from California for the summer.
- Teacher hmm...that's kind of scary. how does the structure seem different? more layers of structure?
- Teacher cool! now someone can answer all of the kids questions instead of google!
- Bugscope Team We think that there is an anti-clumping agent that makes them look like this.
- Bugscope Team Aztec salt.
- Teacher the kids are here now....let me show them how to login in...only two laptops so they are sharing. I also have a projector screen up.
- Bugscope Team cool beans
- 8:32am
- Bugscope Team Hi Kev, and T.I.E.!
- Bugscope Team Let us know when you have questions.
- Student Hi
- Student hello!
- Bugscope Team Katy we can also give control to a student if you would like to give them a chance to drive
- Bugscope Team This is salt -- sodium chloride -- from a Wendy's restaurant.
- Student cant wait to start
- Bugscope Team NaCl
- Student hi T.I.E.
- Student yo kev
- Student what's up?
- Student u r right across from us! u know!
- Bugscope Team OOF
- Bugscope Team better...
- Student yup
- Bugscope Team much better -- you are good at this
- Student out of focus
- Student thank you
- Student this is cool!
- Student what?
- Bugscope Team I am sitting in front of the microscope, Cate is at the computer next to the 'scope, Annie is in Cali...
- Bugscope Team this is salt from Wendys if you didnt already know
- Student kev, stop talking!
- Bugscope Team In California where it is 6:37 am
- Student we knew
- Teacher we're going to take a look at the insects we sent in and then the other cool specimens you provided.
- Bugscope Team this is a so-called environmental scanning electron microscope but we are using it as a normal SEM
- Student the salt looks cool!
- 8:37am
- Teacher we'll go back to that later--we think its cool too.
- Bugscope Team this is your spider
- Student the spider is scary!
- Bugscope Team where the spinnerets are
- Teacher it was in my bedroom!
- Student oh gross!!!!!!!!!!!!!!
- Bugscope Team spider booty
- Student eek!!!
- Teacher hey!
- Student hahaha
- Bugscope Team clearly when the spider is alive this is not shriveled like it is now
- Student ewww!
- Student I killed one yesterday
- Teacher spiders eat mosquitoes which sting me and bother me a lot more than this spider.
- Bugscope Team good that spiderman doesn't have spinnerets
- Student is this where the webbing stuff comes from?
- Student yes
- Bugscope Team yes it is hard to see the spinnerets here with all of the setae around them, but they are the smooth hairlike things
- Student u dont know that, kev!!!
- Bugscope Team kev was right
- Bugscope Team they produce different kinds of web, and some are not sticky
- Bugscope Team the dots are the bases of setae that have broken off
- Student thanks scott
- Bugscope Team you can see that the surface of the body is infolded, quite a bit
- Student spiders have 8 eyes
- Student you can tell were girls
- Bugscope Team and when the spider was alive it would have been expanded
- 8:42am
- Student really?
- Student do u know if this spider is a female or male
Bugscope Team we think it is a female
- Bugscope Team spiders are softbodied -- they are not like most of the insects, which have exoskeletons
- Student oh thanks
- Bugscope Team male spiders have larger palps -- the armlike things in front of the face
- Teacher my controls disappeared
- Teacher there they are
- Student good
- Bugscope Team you can see now that this is where one of the setae had been
- Student what are setae?
Bugscope Team insect hairs
- Bugscope Team spiders, you know, are very sensitive to vibration
- Student is a setae anything like a sautay
Bugscope Team it is pronounced see-tee
- Student thanks
- Student jocking!
- Bugscope Team and the setae (see-tee) are hairlike structures that have (often) sensory functions
- Student thats not how you spell jokeing
- Bugscope Team some setae (singular is seta) are chemosensory and some are mechanosensory -- like cat or rat whiskers
- Student T.I.E can't spell!
- Teacher I heard that they help the insects stick to the wall.
Bugscope Team some setae do. a specific setae called tenent setae, which we have a few presets of today
- Bugscope Team or, as Cate says, mice whiskers, to be fair
- 8:48am
- Student what is the thing in the middle of circle?
Bugscope Team that is a broken off seta (which is a singular setae)
- Bugscope Team yesterday we saw tenent setae on a spider that had finer tiny setae attached to them
- Teacher oh yes! ;) they'd like to look at the spider's eyes, so I'm going to go to that preset and hand over control.
- Student thank you cate
- Bugscope Team geckos have something similar but about a tenth the size
- Bugscope Team Let us know who you would like to confer control to
- Student what is that?
- Bugscope Team the eyes are kind of beat up looking
- Bugscope Team four, and two, and two
- Student this eye is very interesting i would like to lern more about it.
- Bugscope Team you can see 6 eyes in the front, and to eyes on either side on top of the head
- Bugscope Team Kev do you want to drive?
- Teacher Hello, I'm Alex
- Bugscope Team Oh I see.
- Teacher Katy's letting me drive
- Bugscope Team I don't know how much work has been done into how spiders see
- Student no thanks
- Bugscope Team I know that some spiders, like jumping spiders, are visual predators, and apparently can see as well as we can.
- Bugscope Team The eyes, when we have not coated them with gold-palladium and we are not imaging only the surface, seem to have pupils in them like people eyes
- Bugscope Team Katy: You all are asking good questions. One comment: You will have access to all your images via the database after the session.
- Bugscope Team And some spiders, like the ones that spin large elaborate webs, can hardly see at all. They sense their prey by vibration
- Student we had to change our name cause we loggedout
- Bugscope Team that is a disadvantage of SEM -- that we cannot see through things that are normally transparent.
- 8:53am
- Bugscope Team There is a big black widow and a bunch of her babies (I guess) living in an upside down pot outside my house here.
- Bugscope Team so should we ask what TIE or TIEE stands for?
- Student T.I.E the spider is ALIVE! HA HA HA HA HA !!!!!!!!!!
- Bugscope Team Can you save a baby for us?
- Student we just made it up
- Student nice kev
- Bugscope Team black widows like to stay in one place, fortunately
- Bugscope Team They are still pretty big--and I am not 100 sure they are the babies. But, I can try to catch one without getting bitten
- Student a black widow!? i do not want one of those!!!!!!
- Student T.I.E.E. out, I know where you live!
- Student haha kev i live with you!
- Bugscope Team Maybe..I think they are giant babies
- Student I ment look out!
- Bugscope Team they are really beautiful -- they are shiny like wet plastic
- Bugscope Team I hope they don't eat her
- Student what are shiny?
Bugscope Team Black widow females
- Bugscope Team this is some juju on one of the eyes
- Student what is that?
- Bugscope Team black widows are shiny
- 8:58am
- Bugscope Team uh-huh, yes she is really gorgeous. We had one living in the house last year and one in the car too.
- Bugscope Team oh maybe the other ones are boys
- Bugscope Team They are not agressive at all
- Student what is juju?
- Student does the female black widow eat the male black widows?
Bugscope Team If she is hungry and he is not fast enough to get away
- Bugscope Team I have one of those jumping spiders living in my car, in the trunk
- Student oh
- Bugscope Team juju is our word for slime or other unidentifiable debris
- Teacher What is the juju
- Student i hate jumping spiders! why dont you take it out?
Bugscope Team scott like to keep pet spiders
- Bugscope Team Spiders are usually not aggressive at all. They would rather just run away than have to bite you. The venom is expensive to make, and they certainly can't eat you.
- Student oh, thanks.
- Bugscope Team in this case it is some oil or something that is on the eye and charging up with electrons
- Bugscope Team I just had one of my favorite spiders, from this building, die
- Student so do I
- Student hey we asked that question kev!
- Bugscope Team so of course Cate cut it in half and put it on this stub
- Student what is that hair?
- Student why do you keep spiders for pets? do you study them?
- Bugscope Team more sensory setae
- Student thanks
- Bugscope Team you can see one of its claws in the lower left corner
- Student i see it! i see it!
- 9:03am
- Bugscope Team this is the season for bugscope, but it is not what we do all of the time
- Bugscope Team theres some spider fangs
- Student look at those fanges
- Student look out! T.I.E.E.
- Student thats not how you spell fangs
- Student what us SEM, TEMetc.?
Bugscope Team SEM is what we are using now, the Scanning Electron Microscope, and the TEM is another electron microscope we have that stands for Transmission Electron Microscope
- Student is that it's mouth?
- Bugscope Team its hard to see the end of the fangs on spiders. I would love to see the venom pores
- Student i would 2 cate
- Bugscope Team sometimes when a male spider wants to mate with a female spider and not get devoured, he will make a packet of web and stick it on the chelicerae so they cannot open and she cannot bite
- Student what is the chelicerae?
Bugscope Team those are the buck teeth looking things where the fangs are attached
- Student cool
- Bugscope Team we had read about that and then one week we found a female spider with a wad of web stuck right between the chelicerae
- Bugscope Team the chelicerae, or chelicers, are the jaw-like things that the fangs are attached to
- Student is the spider on it's back?
- Student you are me understand cate thank you
- Bugscope Team as Cate says they look like buck teeth
- 9:08am
- Student why are the spiders legs curled up? is it dead?
- Bugscope Team yes it is on its back -- we often mount bugs, etc., on the dorsal side so we can view the ventral side
- Teacher How do we capture images?
Bugscope Team you are capturing images as you are looking at them. All your images are saved as we go along and can be viewed from your teacher page in the transcript
- Bugscope Team yeah sorry it is dead -- one of the spiders you sent was still alive
- Student opps!
- Bugscope Team you can imagine that it would be hard to image a living spider
- Student thanks Scott
- Bugscope Team this is the spider you sent, the dead one
- Student the spider looks squished!
Bugscope Team yeah..the abdomen deflates when they die
- Student is that its butt
- Bugscope Team yes not to put too fine a point on it
- Bugscope Team as Dickens would say
- Bugscope Team oof
- Bugscope Team OOF
- Bugscope Team yay!
- Student what are those two half circles?
- Teacher What is the part we're looking at now?
- Student that's better
- Bugscope Team some of these spinnerets produce sticky web and some produce smooth web
- Student what are the little tiny circles
Bugscope Team those are where the setae broke off
- 9:13am
- Student ?
- Bugscope Team you can tell that the tiny circles are where setae were lost
- Student ok
- Student wow!
- Bugscope Team spiders, if they get stuck in their own web, can eat their way out
- Student yes i can see into the holes
- Student yes cate it is!
- Student thats one way to recycle!
- Student wish we could do that!
- Bugscope Team note that Spiderman does not seem to recycle. And Peter Parker does not have web for breakfast.
- Bugscope Team this is one of the claws; we were thinking that the spider could slide along the nonsticky web with it.
- Bugscope Team spider silk is 5 times as strong as steel
- Bugscope Team it has a built-in comb
- Student you know what else i noticed, scott loves spiderman
- Bugscope Team I just had some web with my yogurt and cereal...mmm
- Bugscope Team not really
- Student good
- Bugscope Team I like Iron Man now.
- Student i want to have that for lunch!
- Student OK
- Student one order of a hamburger and web, please!
- 9:19am
- Student what are we looking at now?
- Bugscope Team on Wikipedia it says that 16 ounces of spider web could circle the world
- Bugscope Team Cool Brett let us know if you have questions.
- Student really?
- Student what is this
- Bugscope Team this is a caterpillar
- Student do spiders have teeth?
Bugscope Team no, they have fangs that they use to inject venom into their prey. The venom liquifies the inside of the prey's body, and the spider sucks down the juices. No chewing involved.
- Bugscope Team spiders have fangs, but they do not chew
- Student we have now become T.I.E.E.K.! (kev joined us!)
- Student spiders have fangs, right?
- Bugscope Team spiders inject venom into their prey that immobilizes them and also liquefies the insides of the prey.
- Bugscope Team spiders drink their prey like a milkshake
- Student How many fangs do spiders have?
- Bugscope Team two
- Student is this the same spider we were looking at a moment ago?
- Teacher Is this the head??
- Student What is the big round part?
- Bugscope Team this is kind of disappointing -- yes it is the head
- Student no its not its a caterpillar
- Student thank you e1
- Bugscope Team it is disappointing because there is a lot of slime on the head that obscures its features
- Student your welcome e2
- Student Who (or what) is e1 and e2?
- 9:24am
- Student is it dead?
Bugscope Team yes, all the bugs in the scope are dead right now
- Student we are sitting across from you.Duh
- Student Katy: speak up!
- Student what would happen if thay were alive?
- Bugscope Team a lot of insects, including the caterpillar forms of insects, release slime that is toxic or tastes bad to discourage other insects, or birds, or especially ants, from eating them
- Bugscope Team it would be hard to pump down the scope to high vacuum if they werent dead, because they are juicy, and even if you did pump it down, the insects would have no air and they would be getting hit with a high energy beam which should kill them
- Teacher Okay, but I am not Katy
- Bugscope Team there is a vacuum in the specimen chamber, so the insects would have to close their spiracles and hold their breath, for awhile...
- Student Do caterpillars have teeth?
Bugscope Team I believe they have a set of hinged jaws
- Student kev you are sitting across the room from him too you have a voice!
- Student hinged jaws?
- Bugscope Team no insects have what we would think of as teeth, but sometimes the jaws are hardened with minerals such as calcium
- Bugscope Team trapjaw ants are said to have harded jaw tips
- Bugscope Team whoops, you drove off the edge of the world
- Student OOF!
- Bugscope Team hardened jaw tips, that is
- Bugscope Team yeah Kev
- Student i had apet caterpiller once. i found it in the bathroom
- Student What are we looking at?
- Student OFF!!!
- Bugscope Team did it grow up into something you recognized?
- Student tot
- Student i mean oof!
- 9:29am
- Student no it died in infancy
- Bugscope Team here is one of its suction cup legs
- Bugscope Team this is like Alien, an alien foot
- Student "Tot" means toddlers out toddling
- Student we are smart you know, it would have grown into a moth or a butterfly
- Bugscope Team or like a squid sucker
- Student What is the thing we are looking at now?
- Student what
- Bugscope Team yeah Cate says it could have been a maggot
- Bugscope Team scott is mean
- Student it looks like one
- Student pbjb
- Bugscope Team this is a deflated proleg
- Student haha cate good one lol
- Bugscope Team a professional leg
- Student kev are you learning anything?
- Student What is the big thing in the middle of the image?
- Bugscope Team that is the proleg
- Bugscope Team of the caterpillar
- Student What is a proleg?
Bugscope Team They are the little squishy legs on the abdomen of caterpillars. They aren't "really" legs, because insects only have six legs.
- Student is that all hair
- Bugscope Team those are setae
- Bugscope Team the hairs are setae
- Student e2 just forgot how to spell hair . e2, i'm a little tierd
- 9:34am
- Student What are the spike things that are sticking out from the proleg?
- Student Haha e2
- Student yeah what is it
- Student Why are you laughing at each other, e1 and e2?
- Teacher Katy says that it is illegal to kill a preying mantis in some states
Bugscope Team I just looked it up: Turns out that's an urban legend and completely false. It has never been illegal to kill a praying mantis in any U.S. state, nor is there any federal protection for mantids. No species of mantid is listed as threatened or endangered in the U.S. There are no fines for killing them.
- Student haha kate
- Student kev, you don't know us! e2 and e1 rock!
- Student your not kev your an imposter!
- Student is that a leg?
- Student Some people are double-jointed; are some spiders double-jointed?
Bugscope Team Nope, spider and insect legs can only bend so far in one direction. No tricks or dislocating or yoga-induced flexibility
- Teacher kev is driving
- Bugscope Team those are called prolegs, as Annie says, because they are insects, and if insects are defined as having six legs then the leg-like things are called prolegs. Sometimes to be an entomologist you have to think like a philosopher.
Bugscope Team hahah
- 9:39am
- Student Thank you, Cate.
- Student What is this? Is it part of the skin?
Bugscope Team this is part of the compound eye of a fruit fly
- Teacher this is a fruitfly eye
- Bugscope Team There are several requirements for being an insect...having six legs groups the class Hexapoda
- Bugscope Team Spiders an choose to eject their legs from their bodies if they want -- for example if they sense poison coursing toward their bodies through a leg they can just let it go
- Student T.I.E.E. just got a new member BB
- Bugscope Team TIEEBBK
- Student Spiders can eject their legs? Do they grow them back?
Bugscope Team Spiders do molt as adults, and so they can usually grow back their legs---so long as there is minimal damage to the tissue at the base of the leg
- Bugscope Team there are setae in between the ommatidia, which are the individual parts of the eye
- Student yescott thats right
- Bugscope Team they don't grow them back
- Bugscope Team uh-oh---I might be wrong.
- Bugscope Team good thing Annie is here
- Bugscope Team she is being super sweet -- she is right
- Student Zoom out
- Student what are the pointy things?
- Student If they don't grow their legs back, how can they survive?
- Bugscope Team Cate says they can molt several times as juveniles but not as adults.
- Bugscope Team really...Pete's tarantulas molt all the time
- Bugscope Team But (Cate says) the tarantula-like spiders live longer and molt as adults.
- Student Daddy Long-Legs grow their legs back though, right??
- 9:45am
- Student who pete?
Bugscope Team Pete is my lab mate who has two or three tarantulas
- Bugscope Team ah-hah! Some spiders (like tarantulas) molt throughout their lives. Some do not. We are both right
- Student who pete
- Bugscope Team Where is Ian?
Bugscope Team Asleep
- Student Katy\Kev: can you zoom out so we can see better
- Student whos ian
- Bugscope Team about DaddyLongLegs, if you look at them up close they are crustacean like
- Student are you all asleep?
Bugscope Team No, Ian, another entomologist, is currently sleeping. SInce it is not 8 am here yet
- Student Hi, Fred
- Guest G'day
- Bugscope Team the controls should work now
- Bugscope Team Looks like the server crashed... Cate had to fit it.
- Student hi there fred
- Bugscope Team here is your ladybug
- Student Kev is back
- Student yuk!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!
- Bugscope Team this ladybug looks like Maria Shriver to me.
- 9:50am
- Student OH YOU MUST BE TIERD
- Bugscope Team wait, that didn't make sense. It is 7:50 am here.
- Student How many lenses do the compound eyes have?
- Bugscope Team Annie is, like, all energy.
- Student e2 is to cant spell
- Bugscope Team I am not too bad, I still haven't really adapted to pacific time. I am a morning person
- Student What are the large things by the mouth? Are they jaws are pinchers?
- Student talk
- Bugscope Team there are two sets of palps -- mandibular and maxillary
- Bugscope Team the things at the top of the screen that are forked are hinged jaws
- Student What exactly are we looking at right now?
- Bugscope Team that help the ladybug manipulate and taste its food, which could be aphids
- Student Does anyone know how many lenses the compound eyes have?
Bugscope Team It varies between species.
- Bugscope Team we are looking into the mouth, as much as possible
- Student no. do you
- Bugscope Team it depends on the insect, of course -- there can be thousands of individual ommatidia
- Student hello, umesh
- Bugscope Team some ants have very few ommatidia
- Bugscope Team Fruit flies apparently have 750 per eye
- Bugscope Team you could easily count them in many ants
- Bugscope Team some ants are blind, too
- Student i mean alot
- Student Oof
- Student where is scot J?
- 9:55am
- Student Who's umesh?
- Student very OOF
- Bugscope Team you can tell when you look at ants that they use their antennae, primarily, to get information about the Outer world.
- Student stillOOF
- Student It looks like swish cheese\
- Bugscope Team click to stop
- Student what?
- Bugscope Team whoever is driving
- Student oofoofoof
- Student ZOOM OUT
- Student guys hes working on it!
- Student going to a new thing
- Student see
- Student hello, anyone there?
- Bugscope Team we might restart the server if we can't get things going...
- Student tEACHER IS WORKING ON IT
- 10:00am
- Bugscope Team the preset apparently moved and the microscope control is angry
- Student are you guye taking a nap?
- Student T.I.E.E. your caps lock is on.
- Bugscope Team we will have to sacrifice a grad student to the microscope to get things working again
- Student not enymore
- Bugscope Team OOF now
- Bugscope Team I am glad I am in CA
- Student OOF OOF OOF
- Bugscope Team i think it should be working again
- Bugscope Team it wouldn't have been Annie -- she keeps us, most of us, in line
- Bugscope Team sorry about that. Our server computer is dying and we are replacing it soon with a mac mini
- Student What is this??
- Student E2 is with Kev
- Bugscope Team this is one of the legs of the mosquito
- Teacher this is emma singing on to drive the crazy bus.
- Bugscope Team you can see the scales
- Student looks like a tree
- Bugscope Team Emma let us know if you need some help.
- Student a very small tree
- Teacher yeah, we are looking at a mosquito
- Student right....
- Student Are these the pads that help the mosquito stick to things?
Bugscope Team no these are scales that help the mosquito slip out of spiderwebs and out of the mouths of birds
- Teacher thanks scott
- Bugscope Team those are wing scales
- Student really?
- Bugscope Team I am not sure if we saw any tenent setae on the mosquito tarsi.
- Bugscope Team presets 19, 10, 8 are all places where insects have the sticky pads
- 10:05am
- Teacher what are the pointy things?
- Student T.I.E.B.B.K.A. now!
- Bugscope Team the pointy things are spines that are likely sensory
- Teacher okay thanks.
- Student Driver: Zoom out, please
- Bugscope Team the driver grayed out but is back now
- Bugscope Team we think they are sensory because they penetrate the cuticle, and thus they likely connect to the nervous system
- Student Emma OOF!
- Bugscope Team these are fruit fly sticky pads
- Bugscope Team these are tenent setae, but they are slightly OOF, as Kev says.
- Bugscope Team looks like a plant
- Bugscope Team looks like here a preset might have moved unless you drove
- Bugscope Team ok we have it back
- Student is Fred asleep, or dead?
- Teacher sorry
- Bugscope Team here is where a mosquito bites you, sometimes we see a lancet poking out
- Bugscope Team yeah this is the tip of the mosquito proboscis
- Bugscope Team only female mosquitos bite
- Student ew
- Student ew
- Student ew
- Teacher i thnk i knew that
- Teacher e1 is short for emma
- Bugscope Team who's e2?
- Student Eve
- 10:11am
- Teacher e2 is eve
- Student e1 move up
- Teacher e2 you are a traitor
- Student this is Kev
- Student How long do mosquitoes live?
- Bugscope Team this is jsut a sheath for the lancet, or stylet, that is inside
- Student e1, im just with kev!
- Teacher not too long i dont think
- Bugscope Team the lancet is very sharp and has serrated edges like a steak knife
- Student yup
- Teacher how dare you!
- Bugscope Team Not very long--there are some that will overwinter as adults. But during the summer, most mosquitoes will live about 2 weeks max
- Student ha
- Bugscope Team the lifespan of mosquitos varies -- some may live only as long as 4 days
- Teacher kev, let my person go!
- Bugscope Team and as Annie says, some females can overwinter and lay their eggs in the spring
- Student no way
- Bugscope Team Fred was probably not sleeping. He has other stuff to do and checks in occasionally to see how we are doing.
- Bugscope Team it's like having the game on, for him
- Student what ire the thingd on the screen part of his legs?
- 10:16am
- Teacher deformed!
- Bugscope Team Kev what?
- Student never mind
- Bugscope Team the legs are lined with scales
- Bugscope Team the wings are made of chitin like the body
- Student what is coming from his mouth
- Bugscope Team cuticle
- Teacher what are those round things
- Bugscope Team the donuts are where its antennae used to be
- Bugscope Team the proboscis projects from the mouth
- Student his face is wierd
- Student What are the round this near its eyes
Bugscope Team those are where antennae used to be attached
- Teacher OOPS!
- Bugscope Team the proboscis is prehensile like a monkey's tail.
- Student are you looking at this bug right now everyone
- Bugscope Team it can probe around and find a good place to bite
- Student Thank you Cate, sorry for the typo.
- Bugscope Team yes Kev.
- Student is he under a microscope?
- Student well duh
- Student You would think that the antennae would be very delicate; how do they not get damaged?
Bugscope Team They are less delicate when the insect is alive. Parts get brittle when the insect dies
- Student what i mean is is he in your room?
- Bugscope Team Kev it is a girl and she is inside a scanning electron microscope
- Teacher scott i think only spider monkeys have prehensile tails
- Bugscope Team we can show you what the chamber looks like now
- Student oh thank you scott
- Teacher okay
- Bugscope Team okay like a spider monkey then
- Teacher much better
- 10:21am
- Teacher cool!
- Student this pic is cool
- Bugscope Team now you should be able to see the inside of the specimen chamber of the 'scope
- Student yes
- Bugscope Team you can see the sample, on a sort of platter that is 1.75 inches across
- Bugscope Team the cone at the top of the image is where the electrons come down the column
- Student does it have to be dark in the room to see the bugs better?
Bugscope Team When we are looking at things in the TEM we have to have it very dark most of the time
- Bugscope Team and the cage to the right top is the secondary electron detector
- Bugscope Team I like it to be dark in the room, but not everyone does
- Teacher Scott, sorry i know everything about mammals. i couldnt help my self
- Student ok that is interesting
- Bugscope Team its ok, you can call out scott when ever you know hes wrong. thats how he learns
- Bugscope Team the electron beam comes down the column and is moved from side to side by scanning coils
- Student what time is it in CA now, cate
- Bugscope Team 8:25am now
Bugscope Team my time
- Bugscope Team so the beam moves like a typewriter, sort of, rastering across the sample
- 10:26am
- Bugscope Team the electron beam is considered primary electrons, and when it hits the sample it produces secondary electrons from the sample surface
- Bugscope Team which is coated with gold palladium
- Bugscope Team looks like the driver is grayed out. we can hand the controls over to a person in the students section
- Student how does katy get back on?
- Student yes but its ok
- Student katy wants controls to go to E1
- Bugscope Team you can log back in as Katy (the teacher) again if you like
- Bugscope Team got it
- Student got what scott?
- Bugscope Team scott gave e1 control
- Student I like my typewriter
- Student How many different types of misquitoes are there?
Bugscope Team according to a website I was on earlier, there are 140 types.
- Student Okay, eve is groovin up to take the controls!
- Student hi y'all! it's e2!
- Student E2 is goingto drive
- Student 140? wow
- Student does anyone like the Beatles?
- Bugscope Team that number could be wrong. That was just according to a site I was at
- Student sorry its taking a little time
- Student that was E1
- Student not Kev
- 10:31am
- Bugscope Team take your time
- Student what are the dots on the eyes?
Bugscope Team the eyes are kind of smooshed, but if the driver wants, she can take the mag up on one of them
- Bugscope Team I used to like the Beatles, some songs more than others, but if I buy a CD I will not listen to it
- Bugscope Team Annie has to log out! I have to go do some research! Have a great rest of the session
- Student traitor!
- Bugscope Team the tiny dots?
- Student that was E1 again
- Bugscope Team brb
- Student what is brb
- Bugscope Team be right back
- Student Be right back
- Student thanks
- Bugscope Team you can increase the mag even more if you want
- Student this is cool
- Student Is there any advantage to having compound eyes??
- Student E1 is falling asleeZZZZ...
- Student that was E!
- Student Imean E1
- Bugscope Team when we do electron microscopy it is easy to forget that the samples we are looking at are frozen in time, and sometimes they are also dehydrated, like now
- Student pop quiz for cate: can you name all of the kinds of mosquitos?!
Bugscope Team no way
- 10:36am
- Student what are the little dots
- Student ?
- Bugscope Team we used to think the little dots were analogous to rods or cones in human eyes.
- Student wow! high mag
- Student Kev if i wanted punctuation i would put it in
- Student What are the advantages or disadvantages of having compound eyes?
Bugscope Team scott is answering it, hold on :)
- Student sorry
- Student apoligie accepted
- Student thanks E1
- Bugscope Team compound eyes give an insect an advantage over us in that they may have better peripheral vision
- Student who hungry?
- Student im talking to myself again
- Student hey!
- Student that was E1
- Bugscope Team they have many lenses instead of just a couple of big ones, and if some of the ommatidia get dirty there are often enough that it does not matter
- Student can flys see color
- Student ?
- Student can flies see all of the colors we can?
- Student ommatidia?
- Student what T.I.E.E.
- Student ?
- Bugscope Team some insects can see more colors than we can
- Bugscope Team ommatidia are the eye facets
- Student what about moqutios?
- 10:41am
- Student what are ommatidia?
Bugscope Team they are the individual parts of the compound eye
- Student ?
- Student hello?
- Student good by
- Bugscope Team Cate found a site that says that compound eyes have essentially a much faster refresh rate -- they can see faster than we can by far
- Student thanks
- Student Driver: can you zoom out so we can look at something different now?
- Bugscope Team Emma is drivin'
- Student zoom out
- Student no, e2 is
- Student its eve
- Student Zoom out so we're not looking at such minute details
- Student im sitting
- Student lookin at a june bug
- Student Out! Zoom out!
- Student emma is sitting next to kev
- Student now lets look at a july bug :)
- Student Emma has bad punctuation
- Bugscope Team each of the ommatidia will get a bit of the "image" and this gets pieced together and made into a "mosaic". Flying insects like mosquitos have very large compound eyes which will give them almost 180 degree view
- Student .
- Student What is that?
- Student what is what
- Student ?
- Bugscope Team a "july" june bug
- Student a June bug
- Student Thanks, Scott
- Student t thought it was a june bug
- Bugscope Team you can see that it has lost the terminal end of one antenna
- Bugscope Team this is the head, the club-looking thing is an antenna
- Bugscope Team it looks much like a june bug
- Student now lets seea "may fly"
- Bugscope Team we don't have mayflies today
- Student are you all looking at the exact thing we are looking at?
- 10:46am
- Bugscope Team haha kev, I get it :p
- Bugscope Team yes we are looking at the beetle -- is that what you see?
- Bugscope Team do you think we would be looking at something different?
- Student what is that thing low center?
- Bugscope Team if not hit refresh
- Student Why does it have only one antenna?
Bugscope Team the other one broke off. Antennae are easy to break, as well as legs, when insects are dead
- Student eww what is it!
- Student no we are looking at a June bug
- Bugscope Team the thing between the forelegs we have seen before but I do not know what it is
- Student that was E1
- Bugscope Team E1 it looks like a june bug but it is small
- Bugscope Team compared to a june bug
- Student T.I.E.E. it probably broke off
- Student exactly what cate said
- Student who is kk?
- Student Pan the view so we can see the rest of the insect, plese
- Student have you seen the movie "the blue butterfly"? it is all about insects
Bugscope Team I have never seen it
- Student what is that
- Student ?
- 10:52am
- Bugscope Team that was some debris on the tarsus of the beetle
- Bugscope Team tarsi, or tarsomeres, are the 'forearm' segments
- Bugscope Team the debris had at least one scale
- Student it is really good
- Student .
- Student What is the fan thing we are now looking at?
- Bugscope Team does the movie have real insects in it?
- Student THEY DONT KNOW
- Student yes. iots
- Bugscope Team not the Jerry Seinfeld kind with only four legs
- Student i mean lots
- Bugscope Team yeah I am sorry I don't know what this is
- Student No not like that
- Student Is kk still here?
- Bugscope Team we have seen them and they seem to be sensory but I don't know what its function is
- Bugscope Team there is more and more to know...
- Student is this part of his mouth?
- Student If you are still here, speak to us.
- Bugscope Team E1 this is something that is part of the thorax
- Student T.I.E.E. i will call you uu!
- Student cool
- Bugscope Team When Cate is not writing she is looking stuff up.
- 10:57am
- Student Which is the most complex part of the insect? The head, thorax, or the abdomen?
- Student hahahahahahaha Katy is tooooooooo!
- Bugscope Team I am not sure we could answer that correctly -- maybe the head
- Bugscope Team tiny bit OOF
- Bugscope Team Junebugs are also known as May beetles. odd
- Student lookin at a butterfly
- Student Emma and Kev are feeling dead
- Student ZZZZZZZZZZZZZZZZZZZZZZZ
- Student what?
- Bugscope Team when we were looking at this before we put it into the 'scope we could see that it reflects yellow, bluegreen, and blue light, depending on the angle at which you hold it
- Student katy just applied the heart starting machine
- Bugscope Team This is a long session, and we have only a 1.75-inch stub of things to look at.
- Student what part of the butterfly are we looking at
- Student The wings always seem to have veins; why is this?
- Student ?
- Student i pity you
- Student vains?
- Bugscope Team this is a closeup of only a couple of scales
- Bugscope Team some of the veins carry hemolymph, which is like blood
- Bugscope Team the scales are analogous to feathers on a bird
- Student butterflys have scales
- Bugscope Team but these are like easily detachable scales
- Student is that what gives them their color?
- Student ?
- Student Insects have exoskeletons, so why would the veins be on the outside?
- Student good question!
- Bugscope Team they seem to have both pigmented and structural colors
- 11:02am
- Bugscope Team there are no veins on the inside
- Student what do you mean by structural colors
- Student this is Katy now btw.
- Bugscope Team they have an open circulatory system
- Student scott you are still a traitor but when my play about the beatle sis famous im sure youll come see it
- Student I see that it is noon, so we don't want to take up all of your friday. I just want to thank you very much--we had soooo much fun and learned so much!!!
- Bugscope Team structural colors are produced by light refraction
- Student By, all. Thanks for your good answers.
- Bugscope Team thanks for all of your great questions
- Student can we all access the images using the password 'katy?'
- Bugscope Team we love bugscope
- Bugscope Team yes E1
- Student thanks for your great answers, guidance, and funny jokes!
- Bugscope Team like if you remember records - the black grooves on the record produce colors that are not from pigments
- Student what a great project
- Bugscope Team just go to your member page and click on the transcript
- Bugscope Team Thank You!
- Student Thank you all for teaching us about Bugscope we all had a lot of fun! from, T.I.E.E.B.B.K.K.A.!
- Student Good by. Thanks you!!!
- Student those are all of our initials
- Student bye!
- Student see ya!
- Bugscope Team Bye!
- Student ;) So long, from sunny western NY!
- Bugscope Team bye T.I.E.E.B.B.K.K.A.!
- Student Bye!
- Bugscope Team See you next year.
- Bugscope Team bye from not so sunny middle of illinois
- Student Thanks Cate!
- Student We really appreciate it and we will DEFINITLEY SEE YOU NEXT YEAR!
- Student see ya Kev
- Bugscope Team yay!
- Student good bye.
- Student Byeeeeeeeeeeeeeeeeeee!!!!!!!!!!!!!!!!!
