Connected on 2007-10-01 12:45:00 from Park Ridge, IL, US
- 12:00pm
- Bugscope Team Tony should be able to get on now, right?
- Bugscope Team I can call him.
- Bugscope Team yep, tony can login now.
- Bugscope Team 1.5 on the vac, real close
- Bugscope Team Cate says 1.5...
- Bugscope Team yes, 1.5
- Bugscope Team scott, can you hear me?
- Bugscope Team d'oh
- Bugscope Team uh no
- Bugscope Team psst
- Bugscope Team oh now I can
- Bugscope Team are we there yet/
- Bugscope Team 1.5 still
- Bugscope Team beetle effect
- Bugscope Team it's had a week though, to dry out
- Bugscope Team guess it would've been worse
- 12:05pm
- Bugscope Team unless we closed a hair (not a seta) in the door
- Bugscope Team is it still 1.5?
- Bugscope Team sheesh
- Bugscope Team so, if a hair gets stuck in the vacuum seal, the symptoms are the vac goes down, but never quite reaches acceptable leves?
- Bugscope Team we are still at 1.5
- Bugscope Team yeah it cannot quite get there
- Bugscope Team 6 of em, yep
- Bugscope Team 1.4
- Bugscope Team close
- 12:11pm
- Bugscope Team dang
- Bugscope Team still 1.4, we'll make the presets fast
- Bugscope Team what's the score now, are we there yet?
- Bugscope Team 1.3....
- Bugscope Team 1.3 intermittent!
- Bugscope Team just waiting for vac okay now
- 12:17pm
- Bugscope Team 1.4 again
- Bugscope Team nope, went back to 1.4
- Bugscope Team should we open and take out some bugs?
- Bugscope Team venting, and trying again
- Bugscope Team hello mrs. dimaso!
- Bugscope Team welcome to bugscope
- Bugscope Team we are setting up still, putting bugs in the scope and waiting for the vacuum, and then we'll make some presets
- Teacher Hello from Dr. Clishem (Tony), Mrs. DiMaso (Caroline) and Mrs. Mitchell (Pat)
- 12:22pm
- Bugscope Team we start at 12:45 right?
- Bugscope Team scott is working on the scope, getting the vacuum set, and then we'll make presets, and then i'll unlock the session, and then you can start!
- Bugscope Team one thing to note when using the Navigation tool, is when you Drive you click once to start moving, then click again to stop.
- Bugscope Team sometimes it is difficult to get the hang of "driving", so if you have problems with it, try using "Click to Center" instead. this will allow you to click once, and the scope will move to that position.
- Teacher 12:45- 12:50 is the start time. I have 28 kids--Should all 28 be allowed to chat or would you prefer that I pair them up to cut down on traffic?
- Bugscope Team well, i think we can handle 28 at a time, but it might get a little hard to answer if we get 28 questions at once. maybe pairing kids up is a good idea? 2 kids to a computer? but, it is up to YOU as the teacher how you want to do it. we will try to accomodate.
- Bugscope Team do you have 28 computers?
- Teacher We'll go with having them work in pairs.
- Bugscope Team okay, sounds cool.
- 12:27pm
- Bugscope Team we are having a little trouble getting the vac down on the scope, so we are a little behind, we will be in time though, for starting at 12:45-12:50.
- Teacher This is Tony . . . I will be leaving half way through the session, but Ms. DiMaso feels pretty confident about the whole thing. The controls look different. Are they easier than before?
- Bugscope Team some bugs have air pockets in them, and so the vacuum doesn't work all the way, so we have to take some bugs out, close the scope again, and try the vacuum again.
- Bugscope Team yes, the interface is much better than before.
- Bugscope Team but keep in mind what i said before about navigation. click to center is easier than driving.
- Bugscope Team you can see now we are openinig the scope to fix the bugs.
- Bugscope Team that's scott
- Bugscope Team now we took out a bad bug and are trying the vacuum again.
- Bugscope Team when controling the scope, just remember there is a slight lag in every operation. when you click on a control like moving or adjusting the focus, try clicking once and wait for the change, and once you get used to the speed of the interface, it does work well.
- Bugscope Team we tried to make the interface as close to what controlling the real ESEM is like.
- 12:36pm
- Bugscope Team okay what's the score now 1.5?
- Bugscope Team we are waiting for 1.3 x 10-4 mBar
- Bugscope Team 1.4
- Bugscope Team so we can bring up the beam and grab the presets
- Bugscope Team a lot of pressure on Cate to get the presets done
- Bugscope Team good thing she is tough
- Bugscope Team Chas rewrote the software, and he has been with us since the very beginning. So he knew what to do...
- Bugscope Team cate is the champ!
- Bugscope Team 1.3
- Bugscope Team 1.3...
- Bugscope Team Here we go.
- Bugscope Team starting the presets. we will be ready in 8-10 minutes
- Teacher Did Chas start in the mailroom only someday to become CEO of the company? --Tony
- Bugscope Team heh. well, not the mailroom exactly, more like started as an intern.
- Bugscope Team Chas started as free labor in high school as a sophomore.
- Bugscope Team now he is a grad student in bioengineering.
- Bugscope Team kind of like Blade Runner.
- Bugscope Team palp
- 12:41pm

- Bugscope Team looks like a jet engine
- Bugscope Team Chas works for the Terrell Corporation.
- Bugscope Team wing
- Bugscope Team heh

- Bugscope Team Tony where are the kids?
- Bugscope Team pollen grayne
- Bugscope Team they said the kids are coming at 12:45-12:50
- Bugscope Team grayne i mean?
- Teacher They are here and we are watching the presets and seeing the chat. They have not logged in yet.
- Bugscope Team tell Cate she's doing a good job.
- Bugscope Team grain

- Bugscope Team you can see brochosomes
- Bugscope Team hi kids, welcome to bugscope!
- Bugscope Team this is odd -- we're giving away all the presets
- Bugscope Team students, i mean.
- Bugscope Team this is a louse, courtesy of one of Kendra's children.
- Bugscope Team juju

- Bugscope Team Yay Claudi is here.
- Bugscope Team Hey everybody
- Bugscope Team Cal
- Bugscope Team you can go ahead and have the students login if you want. we will be ready very soon.
- Bugscope Team Claudi we are doing the presets.
- Bugscope Team one more preset...
- Bugscope Team ARe those bugs sent by the teacher?
Bugscope Team no, these are bugs we had.
- 12:47pm

- Bugscope Team okay, we are ready!
- Bugscope Team WELCOME TO BUGSCOPE!
- Bugscope Team i have just unlkocked the session mrs. dimaso, you can start controlling the scope now.
- Bugscope Team when you have questions, go ahead and ask away
- Bugscope Team we can also give control of the scope to any of your students, if you should want that.
- Bugscope Team alright we are ready to roll
- Bugscope Team What grade is this?
- Bugscope Team Annie is in class today, so she cannot help, but Smiley (Claudi) is here.
- Bugscope Team I will try my best =)
- Bugscope Team Maybe someone can tell us what grade we're working with?
- Bugscope Team grades?
- Student We are in 5th grade.
- Bugscope Team Hi Shelley!
- Student what are we looking at
- Bugscope Team Cool Samanth and Sarah thank you
- Bugscope Team this is the compound eye of some sort of flying insect
- Bugscope Team the individual facets of the eye are called ommatidia.
- Student what is the magnification?
- Bugscope Team Shelley are you working with us tomorrow?
- Bugscope Team you can see the mag a the top right, it is currently 2797X


- Bugscope Team Mrs Dimaso has control of the 'scope now.
- 12:52pm








- Bugscope Team as you can see, those eyes are pretty big
- Bugscope Team now you can see where we were.
- Bugscope Team they take up the entire side of the head
- Bugscope Team looks like this guy has four wings, like a wasp
- Bugscope Team How about contacting one of the drosophila labs...should be easy to do...don't you think Scott?
- Student what is this little creature
- Student what's the white stuff on it's eyes
- Bugscope Team I think it's a small wasp

- Bugscope Team Alex: How do I do that?
- Bugscope Team when you take up the mag a lot on an insect, you will often see small pieces of debris like dirt all over them, some more than others
- Student How did you put it in there
- Bugscope Team smiley, press the tilda key.
- Bugscope Team there is a moire pattern on the eyes
- Bugscope Team should be the key to the left of the number 1
- Bugscope Team Cate made the sample and stuck this little wasp onto the carbon doublestick tape
- Bugscope Team she also stuck it down with silver paint



- Bugscope Team you should be able to see how the hind and fore wings connect.
- Bugscope Team there are tiny hooks there called hamuli.

- Bugscope Team Look at all those fine hairs (setae) on the wings!!!
- 12:57pm

- Bugscope Team They are receptors that allow the insect to better navigate through the air

- Student what are those hooks for
- Bugscope Team some insects with four wings have the ability to hook the hind and forewings together in flight. It makes them more efficient when they fly.


- Bugscope Team my favorite is earwigs.]
- Student how fast can a wasp go

- Bugscope Team focus getting better and better
- Teacher How do I change bugs?
Bugscope Team you can click on a preset, that will take you to another bug, presets are in the lower right corner
Bugscope Team if you can't see the presets, try expanding your browser window to full screen (F11).
- Student are the little hairs on the bug painful if you touch it
Bugscope Team Some of the setae might be so fine and delicate that we may not even feel it through our skin.
- Student is the stinger still in the wasp
- Bugscope Team the hairs (setae) are so tiny you cannot feel them

- Bugscope Team you can, Mrs D, select a new preset from those on the right
- Bugscope Team you can look and see if you want by driving down


- Student Why are the hairs spirel?

- Student do most bugs have hair
Bugscope Team yes, most bugs have hair, which we call setae, those setae help the bugs to sense their surroundings.
- Student how many hairs are in the wing
Bugscope Team There are likely millions of them. But if I am not mistaken the number of setae may vary between different insects
- Student what are the white things on the wings?
Bugscope Team those would be brochosomes, which is something only leafhoppers make
- Bugscope Team spiral hairs do not flew as much in a particular direction -- the spiral shape makes them more rigid.
- Bugscope Team insects really rely on those setae a lot so they can sense their environment, they have hard shells (exoskeletons) and not soft skin like us
- Student average
- 1:02pm
- Student what do they eat?


- Student what bug do u like the most
- Student do the hairs grow
Bugscope Team nope, they are fixed.
- Student what are those things stiking out of them

- Student what type of bug is that?
Bugscope Team this is a louse.
- Student What are those little tiny things


- Student do the presets have teeth
Bugscope Team Presets are favorite sites the bugscope team marked, so that your teacher canjust click on them, to look at it
- Student whatisalouse
- Student Why are the hads have cringes in the arms

- Student what is the stuff coming out of the louse
Bugscope Team Setae...is what "comes out" off the louse
- Student what does they are fixed mean (how are they fixed)
Bugscope Team they are attached to muscles and nerves under the shell, so they don't grow or move, but they can fall off sometimes.
- Bugscope Team this is a louse, from small children
- Bugscope Team a louse is what the word lousy comes from
- Bugscope Team plural is 'lice'


- Student in real life how big is the louse
Bugscope Team Probably about the size of a pencil tip, anybody if I am wrong please correct me.
- Bugscope Team lice are small bloodsucking arthropods that get in your hair

- Bugscope Team it's only a few mm long, very small

- Student does the lice hurt them

- Student do they lay eggs
- Bugscope Team some of the setae extend through the exoskeleton and are attached to nerves
Bugscope Team Yes
- Bugscope Team lice just makes your head itch
- Bugscope Team they lay eggs on your head, and new ones hatch out
- Bugscope Team they lay eggs in your hair with some gooey stuff so they don't fall off
- Student how do you get lice
Bugscope Team For example from someone who has lice

- Student do the louse have hair on it
Bugscope Team yes, lice do have hairs (setae) as well.


- Student How do they put the lice on the microscope, where did you get them?
Bugscope Team all bugs have to be preped before they go into the microscope, that involves coating the bug with gold palateum, and then taping the bug to a stage, and then putting it in the scope and turning on the vacuum.

- 1:07pm
- Student is part of that leg cut off
- Bugscope Team you can catch them from kids that have them -- if the kids have not come from a clean environment

- Bugscope Team some households are not as clean as others
- Student where do the lice live beside's hear
Bugscope Team There are different types of lice, some live on people, and then I think there are lice that live on trees. The ladybugs eat those.
- Student whats the gooey stuff
Bugscope Team it's their own saliva
- Bugscope Team lice have features that allow them to attach to hair on your body
- Student what is the stuff piling on the louse


- Student why is there so little hairs



- Bugscope Team like they have little clinging limbs that grasp human hairs
- Student what do they eat
Bugscope Team lice will eat dead skin, or even blood. the color of a louse can change slightly depending on what it is eating. if it eats blood it will become darker.
- Student how many bugs do you guys look at a day
Bugscope Team I work with Praerie voles (mammal)...but help out with Bugscope...that's when I look at bugs.

- Bugscope Team we do not always look at insects and arthropods
- Bugscope Team just, mostly, for bugscope sessions like this
- Student what kind of bug is that
Bugscope Team Firefly
- Student is that hair too
Bugscope Team well, yes, it's hair or setae.


- Bugscope Team or lightning bug whichever you call it

- Bugscope Team insects tend to be a lot hairier than you would think

- Student where do the light come from
Bugscope Team do you mean in the image? the image is taken by gathering electrons that are being shot off the bug inside the microscope.

- Student in the bug
- Bugscope Team we are using electrons that come down from above the sample
- 1:12pm


- Bugscope Team the electrons arrive in an electron beam





- Bugscope Team they glow because of a chemical reaction with their organs
- Bugscope Team and they scan continuously across the sample
- Student no the light in the bug that u see at night
Bugscope Team ah sorry, yes, cate says it's a chemical reaction that causes the firefly to light up.
- Student where dose it glow on this fire fly
- Bugscope Team yeah the light in the lightning bug comes from the abdomen, sorry
- Student Are the things coming out of it hair
Bugscope Team the light is coming from within it, from a chemical reaction with their organs

- Student what is the spickes with the balls on top
- Bugscope Team you'll notice the brochosomes on here too
- Bugscope Team scott is controlling the scope, focusing.
- Student where is the light on the bug
- Bugscope Team those small balls are called brochosomes
- Student What are the little white balls
Bugscope Team those are called brochosomes
- Student wow
- Bugscope Team I don't know much more than that
- Student what does the claw do????
Bugscope Team i think claws can do all kinds of things for the bug, like scoop food into it's mouth for example.


- Bugscope Team the brochosomes are something only leafhoppers produce
- Bugscope Team brochosomes are small waxy balls, sometimes shaped like footballs, that leafhoppers put on their bodies, and on their eggs to keep them from drying out.

- Student what type of microscope are you using
Bugscope Team this are images from an Environmental Scanning Electron Microscope (ESEM).

- Student how many of those balls do the bug have
- 1:18pm
- Student are the bugs frozen?
Bugscope Team Before we can use them with the microscope, they must be dried
- Teacher Can you focus on the white ball for us!
Bugscope Team i can't seem to focus on it, too high of a mag maybe?
- Student where do you get all the bugs
Bugscope Team Backyard, on the way home from work, whereever you can find bugs...
- Bugscope Team scott is working on the mag now.
- Student What are the little circles in the ball's?
- Student what are those line things
Bugscope Team I think they are setae...at least I hope that's what you mean by line things
- Student How big is the bug
- Bugscope Team it's kind of like a whiffle ball
- Bugscope Team you've seen a firefly before right? it is about an inch big
- Bugscope Team okay I am back

- Bugscope Team the balls are sticking to its "hair" or what we call setae (seta for singular)
- Student What does the light on the firefly feel like?
- Bugscope Team that big ball right there is a grain of pollen
- Bugscope Team this is cool -- this is a pollen grain, much larger than the brochosomes
Bugscope Team Wow...I did not know that pollen grains can have different shapes.

- Bugscope Team this is on the wing of a lacewing bug
- Bugscope Team the firefly light is cool to the touch
- Student what is a lacewing???

- Bugscope Team those are those green bugs with long transparent wings

- Student whats the hairy things on its leg



- Student what is the smallest bug u have and can we look at it later

- 1:23pm



- Student is it a leaf

- Student Is that a leaf or a wing
- Bugscope Team this is a wing
- Bugscope Team some of them can bite people, although they are too small to eat them
- Bugscope Team and it looks very hairy
- Student what are the bubbles on the outside
Bugscope Team Bubbles in the carbon tape, used to afix the bugs to the little holder which is placed inside the microscope
- Bugscope Team this is the wing
- Student what are the lines and cracks?

- Bugscope Team the bubbles are dirt or grease

- Bugscope Team the lines are the veins of the wing

- Bugscope Team sort of like the wooden sticks that are the rigid members of a kite
- Student isit a leaf bug?

- Bugscope Team this is a lacewing -- a flying predatory insect
- Bugscope Team it eats other bugs

- Bugscope Team it's not a leaf bug as in a leaf mimic
- Student why do the wings have viens
- Student Why are some parts of the wings darker then other parts?
- Bugscope Team the veins support the structure of the wings


- Bugscope Team some parts send less signal to the secondary electron detector
- Student what is this ball ror
Bugscope Team The ball is pollen. Many plants depend on pollen to more plants to fertilize flowers to produce fruits such as appleas, cherries.





- Student what are the smaller balls around the grai of pollin?
- Bugscope Team when we go to super high mag like this it is difficult to get good resolution because we are not very close to the sample -- we are nearly an inch away inside the 'scope
- Student what are the dots on the bug
- 1:28pm
- Student dont viens hold blood and y would a wing need blood if it's flat
- Student What is the fuzzy stuff on the bug, why is it fuzzier on that big line
- Bugscope Team bees carry pollen from flower to flower

- Bugscope Team the fuzzy stuff was tiny setae called microsetae

- Bugscope Team sometimes the microsetae form a pattern or a color



- Bugscope Team we think they also provide surface area to give the wing more lift in the air as the insect flies

- Student how did you get the job
Bugscope Team most of us went to college and have degrees in science or related fields, and we are all interested in microscopes and imaging.

- Bugscope Team I started out as an English major and then added biology

- Student when did you know you wanted to be a scienetst
- Bugscope Team I started doing electron microscopy when I was almost out of college.
- Student what experemts r u working on
- Student What kinds of stuff do you do that is fun?
Bugscope Team Bugscope with classes like you =) I like sharing science with others.
Bugscope Team i like playing cards with friends, reading cool books about science fiction, riding my bike, etc.

- Student Did you like bugs as a kid?
Bugscope Team To be honest, I like bugs under the microscope much better. It is amazing to see the structures that you could not see with your eyes alone.
- Student What grade did you start to like science?
- Bugscope Team I liked bugs as a kid
- Bugscope Team I just liked science in general and ended up choosing physics as my major, I got this job from a friend of a friend
- Bugscope Team Cate is a nerd.
- Bugscope Team I've always liked science, some bugs kind of creep me out, but only the live ones, I can handle them when they are dead
- Student
- 1:33pm
- Bugscope Team I read, go to art museums, sometimes collect chairs
- Student why are the hairs on the bug
Bugscope Team the hairs are called setae, they are for helping the bug to sense its environement.
- Student sports
- Student Are those hairs on the bugs?
- Bugscope Team the hairs (setae) allow the bug to feel or smell its environment through its exoskeleton
- Student what is that light grey thing
- Bugscope Team what kind of sports? basketball, baseball, football?
- Student why is part of the bug white and powdery?
- Student how many legs does it have
- Bugscope Team bugs have their skeleton on the outside, sort of like armor
- Student evertthing
- Student How many cells are in this bug? We looked at 1 cell.
- Bugscope Team so they need to be able to sense their surroundings through the chitin, or cuticle
- Bugscope Team there are thousands of cells even in something so small
- Student what is your favorite bug?
- Student what is the smallest bug u ever looked at????
Bugscope Team Mites living on an earwg
- Bugscope Team earwigs are my favorite because they often have mites
- Student thank you
- Bugscope Team Scott is right, there are a lot of cells making up some of the long setae you saw earlier
- Student Thankyou Goodbye.
- Bugscope Team mites are not strictly insects, but they are very small
- Student Thank you soo much we really appriciate it.
- Student are they blind?
- Student thanks and good bye
- Bugscope Team Oh you are leaving?
- Bugscope Team great questions
- Student Thank You!goodby!
- Bugscope Team thanks guys
- Bugscope Team Bye...it was fun
- Student Thank you, good bye.
- Student thank you for your anwers, good bye
- Student Thanks for answering all our questions! Bye!
- Bugscope Team Thank YOU!
- Teacher Our 5th Graders LOVE science and want to thank all of the scientists for showing us these cool bugs!! Thanks!!!
- Bugscope Team Bye, see you next year?
- Student thank u very much, Phil and Justin and that was a awsome thing to look at!
- Bugscope Team you are very welcome!
- Bugscope Team We always have fun doing this and look forward to seeing you again.
- Student bridget wants to be a bugologest
Bugscope Team cool!
- Student Thank you a lot.
- Student thank you for taking all of our Qs bye
- Teacher We do bugscope every year! See you next fall!
- Guest What kind of insect are we looking at?
- Bugscope Team a bugologist is also called an entomologist
- Bugscope Team this is a beetle
- Bugscope Team this is a beetle we found in our hallway
- Student thanks for tanking time to talk to us bye
- Student see you guys next year
- Bugscope Team outside the lab
- Bugscope Team see you next year then, keep it real!
- Bugscope Team sap let us know if you would like to drive the 'scope
- 1:38pm
- Student thank u very much goodby
- Bugscope Team I just gave you control.
- Bugscope Team So sap you should now be able to see that you have presets to the right and controls to the upper right.
- Bugscope Team mrs. dimaso, do you have any remaining question? you can view the entire transcript of your session at your member page, it also has pictures from the session: http://discord.itg.uiuc.edu/members/2007-051
- Bugscope Team please try something like changing the mag or clicking on a preset
- Teacher Thanks! We're done for today! We will look forward to reading the session script!!!!
- Bugscope Team Shelley are you there?
- Bugscope Team Thanks Mrs DiMaso!
- Bugscope Team thank you mrs/ dimaso, talk to you later, and see you next year too!
- Bugscope Team Hi Ben.
- Bugscope Team Shelley you are free to drive if you would like.
- 1:44pm
- Bugscope Team or sap you are free to drive.
- Bugscope Team shelley might be away from keyboard for a moment...
- Guest thanks, WE'll see u tomorrow.
- Bugscope Team shelley = sap?
- Bugscope Team okay, see you tomorrow!
- Bugscope Team well, that's it, closing the session.
- Bugscope Team people, i'm closing the session,l any objections?
- Bugscope Team cate: i concure.